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MABC1558-100UL Anti-SPMD3 (nSMase2) Antibody, clone 14G5.1

Anti-SPMD3 (nSMase2), clone 14G5.1, Cat. No. MABC1558, is a mouse monoclonal antibody that detects Sphingomyelin phosphodiesterase 3 (sSMase) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-SPMD3 (nSMase2) Antibody, clone 14G5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: SMPD3 UniProt Number: Q9NY59
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MABC1558-100UL
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, M, HWB, IH(P)MUnpurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1558-100UL
DescriptionAnti-SPMD3 (nSMase2) Antibody, clone 14G5.1
Alternate Names
  • Sphingomyelin phosphodiesterase 3
  • EC: 3.1.4.12
  • Neutral sphingomyelinase 2
  • nSMase-2
  • nSMase2
  • Neutral sphingomyelinase II
Background InformationSphingomyelin phosphodiesterase 3 (UniProt: Q9NY59; also known as EC: 3.1.4.12, Neutral sphingomyelinase 2, nSMase-2, nSMase2, Neutral sphingomyelinase II) is encoded by the SMPD3 gene (Gene ID: 55512) in human. SMases are classified based on their pH optimum into acid, neutral, and alkaline subtypes. Four different neutral SMases have been described in mammals and nSMase-2 is shown to be the most predominant in cellular systems. nSMase-2 is a phosphoprotein whose activity is regulated by calcineurin (PP2B) and it is shown to be exclusively phosphorylated on serine residues. It is predominantly expressed in the brain and its levels are shown to be up-regulated during G0/G1 phases. Two isoforms of nSMase-2 are reported that are produced by alternative splicing. nSMase-2 catalyzes the generation of bioactive lipid ceramide through the hydrolysis of the membrane lipid sphingomyelin and specifically hydrolyzes the phosphocholine-headgroup from sphingomyelin. It does not exhibit phospholipase C-type activity against phosphatidylcholine, lysophosphatidylcholine, platelet activating factor or lyso-platelet activating factor. For its activity a neutral pH and divalent cations (Mg2+ or Mn2+) are required and its activity is stimulated by the presence of phosphatidylserine (PS) and unsaturated fatty acids. NSMase-2 undergoes palmitoylation and palmitoylation-deficient protein is target for lysosomal degradation. (Ref.: Filosto, S., et al (2010). J. Biol. Chem. 285 (14); 10213-10222; Shamseddine, A.A., et al (2013). Adv. Enz. Regul. 57: 24-41).
References
Product Information
FormatUnpurified
PresentationMouse monoclonal antibody in tissue culture supernatant without azide.
Quality LevelMQ100
Applications
ApplicationAnti-SPMD3 (nSMase2), clone 14G5.1, Cat. No. MABC1558, is a mouse monoclonal antibody that detects Sphingomyelin phosphodiesterase 3 (sSMase) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPMD3 (nSMase2) in human brain tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SPMD3 (nSMase2) in human stomach and human fallopian tube tissue sections.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal region of human Neutral sphingomyelinase 2.
Clone14G5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 14G5.1 detects neutral sphingomyelinase 2 in human and murine brain tissue. It targets an epitope within 101 amino acids from the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMPD3
Purification MethodUnpurified
UniProt Number
Molecular Weight~70 kDa observed; 71.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SPMD3 (nSMase2) in mouse cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1558-100UL 04054839431951