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MABC565 Anti-SPOP Antibody, clone 9B7.1

Anti-SPOP Antibody, clone 9B7.1 is an antibody against SPOP for use in Western Blotting, Immunohistochemistry.

Anti-SPOP Antibody, clone 9B7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: SPOP UniProt Number: O43791
MABC565
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC565
DescriptionAnti-SPOP Antibody, clone 9B7.1
Alternate Names
  • Speckle-type POZ protein
  • SPOP
  • HIB homolog 1
  • Roadkill homolog 1
Background InformationSpeckle-type pox virus and zinc finger (POZ) protein, also known as HIB homolog 1 or Roadkill homolog 1, is encoded by the SPOP gene (also known as TEF2 or BTBD32) in human (Entrez Gene ID 8405). SPOP protein is a component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex and mediates recruitment of target proteins containing the SPOP-binding consensus (SBC) motif. The 42 kDa SPOP protein comprises an N-terminal MATH domain, a bric-a-brac, tramtrack and broad complex (BTB)/POZ domain, a 3-box domain, and a C-terminal nuclear localization sequence. The MATH domain is primarily involved in substrate recognition/binding. Known substrates with SBC motifs include Macro H2A, Puc (Puckered), Daxx (Death domain–associated protein), and Gli (Glioma-associated oncogene). Prostate and endometrial cancers are frequently identified with SPOP mutations in the meprin and TRAF (Tumor necrosis factor receptor-associated factor) homology (MATH) domain, which can potentially lead to altered substrate binding activities. (PMID 25058385). The SPOP protein sequence is 100% conserved among human, murine, and rat (UniProt O43791, Q6ZWS8, and B2RYD9, respectively) species. Cat. No. MABC565, Anti-SPOP, clone 9B7.1, is a purified mouse monoclonal antibody. A GST fusion protein containing the full-lenght human SPOP sequence was used as the immunogen. The binding epitope of clone 9B7.1 has not yet been mapped.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-SPOP Antibody, clone 9B7.1 is an antibody against SPOP for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SPOP in human liver and human kidney tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human SPOP.
Clone9B7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat, Mouse based on 100% sequence homology. Other homologies: 100% homologus to mouse and rat..
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SPOP
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~42 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SPOP in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC565 04055977164046