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DP02 Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416)

This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of SV40 T Antigen (Ab-2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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DP02
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Sv40 Infected CellsMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
DP02-200UG Glass bottle 200 μg
Description
OverviewRecognizes ~94 kDa SV40 large T antigen in SV80 and SV-T2 cells.
Catalogue NumberDP02
Brand Family Calbiochem®
References
ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
Whyte, P., et al. 1988. Nature 334, 124.
Mitchell, P.J., et al. 1987. Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
Sarnow, P., et al. 1982. Cell 28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10, 591.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative control3T3 cells
Positive controlSV80 or SV-T2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections
Application NotesAffinity Purification (use Cat. No. DP02A)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
Paraffin Sections (see application references)
Application CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified SV40 large T-antigen
EpitopeWithin amino acids 83-128
ClonePAb416
HostMouse
IsotypeIgG2a
Species Reactivity
  • Sv40 Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
DP02-200UG 07790788053734

Documentation

Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) Certificates of Analysis

TitleLot Number
DP02

References

Reference overview
DeCaprio, J.A., et al. 1988. Cell 54, 275.
Whyte, P., et al. 1988. Nature 334, 124.
Mitchell, P.J., et al. 1987. Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
Sarnow, P., et al. 1982. Cell 28, 387.
Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J. 1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10, 591.

Citations

Title
  • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
  • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision06-July-2007 JSW
    ApplicationAffinity Purification (use Cat. No. DP02A)
    Immunoblotting (1-5 µg/ml, see application references)
    Immunofluorescence (1-5 µg/ml)
    Immunoprecipitation (1-5 µg antibody, use Cat. No. DP02A)
    Paraffin Sections (see application references)
    DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen.
    BackgroundSV40 T Ag (Ab-2) is a mouse monoclonal antibody with specificity for antigenic determinants unique to the SV40 large T antigen and non-reactive with SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
    HostMouse
    Immunogenpurified SV40 large T-antigen
    EpitopeWithin amino acids 83-128
    ClonePAb416
    IsotypeIgG2a
    SpeciesSV40 infected cells
    Positive controlSV80 or SV-T2 cells
    Negative control3T3 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThe agarose conjugate (Cat. No. DP02A) is also available and suitable for affinity purification and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesDeCaprio, J.A., et al. 1988. Cell 54, 275.
    Whyte, P., et al. 1988. Nature 334, 124.
    Mitchell, P.J., et al. 1987. Cell 50, 847.
    Dixon, R.A.F. and Nathans, D., 1985. J. Virol. 53, 1001.
    Simanis, V. and Lane, D.P. 1985. Virol. 144, 88.
    Mann, R.S. and Carroll, R.B. 1984. Virology 138, 379.
    Sarnow, P., et al. 1982. Cell 28, 387.
    Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
    Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
    Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
    Tooze, J. 1973. Cold Spring Harbor, New York.
    Tegtmeyer, P. 1972. J. Virol. 10, 591.
    Citation
  • Sujatha Radhakrishman, et al. (2004) Intracellular Approach for Blocking JC Virus Gene Expression by Using RNA Interference During Viral Infection. Journal of Virology 78, 7264-7269.
  • Luigi Ricciardiello, et al. (2003) Induction of chromosomal Instability in Colonic Cell by the Human Polyomavirus JC Virus. Cancer Research 63, 7256-7262.
    		•
    Application referencesImmunoblotting Radhakrishnan, S., et al. 2004. J. Virol. 78, 7264. Ricciardiello, L., et al. 2003. Cancer Res. 63, 7256. Original Clone Harlow, E., et al. 1981. J. Virol. 39, 861. Paraffin Sections Sabatier, J., et al. 2005. J. Clin. Pathol. 58, 429. Del Valle, L., et al. 2004. J. Virol. 78, 3462. Del Valle, L., et al. 2001. Cancer Res. 61, 4287. Masumori, N., et al. 2001. Cancer Res. 61, 2239.