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ABN421 Anti-Senataxin (OY11) Antibody

This Anti-Senataxin (OY11) Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Senataxin .

Anti-Senataxin (OY11) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: SETX, ALS4, KIAA0625, SCAR1 UniProt Number: Q7Z333
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ABN421
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN421
DescriptionAnti-Senataxin (OY11) Antibody
Alternate Names
  • Probable helicase senataxin
  • Senataxin (OY11)
  • Amyotrophic lateral sclerosis 4 protein
  • SEN1 homolog
  • Senataxin
Background InformationProbable helicase senataxin (UniProt Q7Z333; also known as Amyotrophic lateral sclerosis 4 protein, SEN1 homolog) is encoded by the SETX (also known as ALS4, AOA2, SCAR1, bA479K20.2) gene (Gene ID 23064) in human. Senataxin is implicated in both genome and RNA transcriptome homeostasis. Senataxin contains a helicase domain for mediating RNA interaction and an N-terminal domain for critical protein interactions. Senataxin is reported to colocalize with DNA repair proteins, including the DNA damage response protein 53BP1, at distinct nuclear foci in S/G2-phase human cells, and the number of these foci increases in response to impaired DNA replication or DNA damage. Senataxin is shown to resolve RNA/DNA hybrids (R loops) that arise during transcription, which can lead to genome instability unless they are resolved efficiently.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Senataxin (OY11) Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Senataxin .
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Senataxin from HeLa, U2OS, and HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin and siRNA-mediated Senataxin downregulation in HEK293 cells (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flag-tagged Senataxin in anti-Flag immunoprecipitates from transfected HEK293 cells expressing Flag-tagged Senataxin (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Senataxin in HEK293 cell lysates (Courtesy of Dr. Kanagaraj Radhakrishnan and Dr. Stephen C. West, The Francis Crick Institute, London, UK).
Biological Information
ImmunogenA mixture of four KLH-conjugated linear peptides corresponding to sequences from both N- and C-terminal halves of human senataxin.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SETX
  • ALS4
  • KIAA0625
  • SCAR1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~300 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Senataxin in 10 µg of HUVEC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN421 04055977297355

Documentation

Anti-Senataxin (OY11) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Senataxin (OY11) Antibody Certificates of Analysis

TitleLot Number
Anti-Senataxin (OY11) - 3172408 3172408
Anti-Senataxin (OY11) - 3204864 3204864
Anti-Senataxin (OY11) - 3425419 3425419
Anti-Senataxin (OY11) - 3934809 3934809
Anti-Senataxin (OY11) -Q2618545 Q2618545

Brochure

Title
Neuroscience Solutions for productive research
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

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Title
Post-Translational Modifications