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MABT1557-25UG Anti-Septin 7 Antibody, clone 19A4

Anti-Septin 7, clone 19A4, Cat. No. MABT1557, is a rat monoclonal antibody that detects Septin-7 and is tested for use in Immunoprecipitation and Western Blotting.

MABT1557-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1557-25UG
DescriptionAnti-Septin 7 Antibody, clone 19A4
Alternate Names
  • SEPT7
  • Septin-7
Background InformationSeptin 7 (UniProt: F6UVF8; also known as SEPT7, Septin-7) is encoded by the SEPTIN7 gene (Gene ID: 100070492) in horse. Septins are highly conserved, GTP-binding cytoskeletal proteins that are involved in various cellular processes, including cytokinesis, membrane dynamics, cellular morphology, carcinogenesis, and neurodegeneration. Thus far 13 different septins have been reported in mammals. They can self-assemble and form filaments that serve as scaffolds for protein recruitment. Their assembly consists of hexamers and octamers that are formed by septin subunits, all including the essential group member Septin 7. Septin 7 can combine with other septins to form heteropolymers and it is a core component of these multimeric septin complexes. These heteropolymers can form a diverse array of higher order structures, including filaments, gauzes, and rings. Septin 7 is shown to be a novel regulator of neuronal calcium homoeostasis. It is widely distributed in the brain and is reported to be the most common septin in post-synaptic density fractions. It affects cytosolic calcium levels by down regulating the expression of the Orai and IP3 receptors. Lack of septin 7 can lead to loss of other septin proteins in homo- and heterooligomeric complexes. It is also shown to be essential for regulation of dendrite branching and dendritic spine morphology. Reduced levels of septin 7 have been reported in blood-derived lymphocytes in an equine model of recurrent uveitis (ERU) that affects inner eye and leads to vision loss. Dedicator of cytokinesis 8 (DOCK8) protein is shown to be an interaction partner of septin 7 in primary lymphocytes and its levels also show a significant decline in models of ERU. (Ref.: Wang, X., et al., (2018). J. Cell. Mol. Med. 22(7); 3298-3307; Schauer, M., et al. (2018). Sci. Rep. 8; 12332).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Septin 7, clone 19A4, Cat. No. MABT1557, is a rat monoclonal antibody that detects Septin-7 and is tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Septin-7 in Immunoprecipitation applications (Schauer, M., et. al. (2018). Sci Rep. 8(1):12332).

Western Blotting Analysis: A representative lot detected Septin-7 in Western Blotting applications (Schauer, M., et. al. (2018). Sci Rep. 8(1):12332).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA linear peptide corresponding to 19 amino acids from the C-terminal region of equine Septin 7.
EpitopeC-terminus
Clone19A4
Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 19A4 is a rat monoclonal antibody that detects Septin 7 protein. It targets an epitope within 19 amino acids from the C-terminal region.
IsotypeIgG2cκ
Species Reactivity
  • Horse
  • Human
Species Reactivity NoteHuman, Horse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SEPTIN7
Purification MethodProtein G purified
UniProt Number
Molecular Weight~48 kDa observed; 49.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human lymphocytes.

Western Blotting Analysis: 1 µg/mL of this antibody detected Septin-7 in human lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1557-25UG 04061842070060

Documentation

Anti-Septin 7 Antibody, clone 19A4 Certificates of Analysis

TitleLot Number
Anti-Septin 7, clone 19A4 - Q3457400 Q3457400

References

Reference overviewPub Med ID
Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
Schauer M, Kleinwort KJH, Degroote RL, Wiedemann C, Kremmer E, Hauck SM, Deeg CA.
Sci Rep  8(1)  12332  2018

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