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MABE974 Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1

This Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Ser/Thr-protein kinase Nek2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NEK2, NEK2A, NLK1 UniProt Number: P51955
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MABE974
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE974
DescriptionAnti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1
Alternate Names
  • Serine/threonine-protein kinase Nek2
  • Ser/Thr-protein kinase Nek2
  • HSPK 21
  • NimA-like protein kinase 1
  • Never in mitosis A-related kinase 2
  • NimA-related protein kinase 2
Background InformationSerine/threonine-protein kinase Nek2 (EC 2.7.11.1; UniProt P51955; also known as HSPK 21, Never in mitosis A-related kinase 2, NimA-like protein kinase 1, NimA-related protein kinase 2, Protein phosphatase 1 regulatory subunit 111) is encoded by the NEK2 (also known as NEK2A, NLK1, PPP1R111, RP67) gene (Gene ID 4751) in human. Nek2 is a member of the never in mitosis A- (NIMA-) related family of serine/threonine kinases. Nek2 is involved in cell cycle regulation, gene expression, and the maintenance of centrosomal structure and function. Cellular Nek2 levels are tightly regulated throughout the cell cycle with noncentrosomal Nek2 expression peaks from S to G2 phase, while cytoplasmic Nek2 is highest in G1, and nuclear Nek2 is highest during S and G2 phase when it is needed for centrosomal function regulation. During interphase, centrosomes are held together by a proteinaceous linker that connects the the mother and daughter centriole. This linker is disassembled at the onset of mitosis in a process known as centrosome disjunction. The mammalian sterile 20-like kinase 2 (Mst2) and the scaffold protein Salvador (hSav1) are shown to directly interact with Nek2A and regulate its ability to localize to centrosomes, where Nek2A mediating linker dissociation by phosphorylating the centrosomal linker proteins C-Nap1 and rootletin. In addition, Nek2 is also reported to regulate gene expression by phosphorylating the splicing factor SRSF1. Nek2 expression is found upregulated in a wide variety of neoplasms including diffuse large B cell lymphoma and multiple myeloma.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Ser/Thr-protein kinase Nek2.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected Ser/Thr-protein kinase Nek2 in 10 µg of MOLT-4 and HL-60 cell lysates.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Ser/Thr-protein kinase Nek2 in HeLa, NIH/3T3, and HUVEC cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal of human Ser/Thr-protein kinase Nek2.
EpitopeN-terminal half
Clone5D2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with all four spliced isoforms of human Ser/Thr-protein kinase Nek2.
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEK2
  • NEK2A
  • NLK1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~52/44 kDa observed (51.8 kDa/Nek2A (isoform 1), 44.9 kDa/Nek2B (isoform 2), 38.8 kDa/isoform 3, 50.9 kDa/Nek2C (isoform 4 or Nek2A-T), calculated). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ser/Thr-protein kinase Nek2 in 10 µg of THP-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE974 04055977181623

Documentation

Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Ser/Thr-protein kinase Nek2 Antibody, clone 5D2.1 Certificates of Analysis

TitleLot Number
Anti-Ser/Thr-protein kinase Nek2, clone 5D2.1 - 3321807 3321807
Anti-Ser/Thr-protein kinase Nek2, clone 5D2.1 -Q2586394 Q2586394

Technical Info

Title
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White Paper: Further considerations of antibody validation and usage.