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07-131-AF488 Anti-Sirt1 (Sir2) Antibody, Alexa Fluor® 488 Conjugate

This Anti-Sirt1 (Sir2) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Sirt1, Sir2l1 UniProt Number: Q923E4
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07-131-AF488
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-131-AF488
DescriptionAnti-Sirt1 (Sir2) Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • NAD-dependent protein deacetylase sirtuin-1
  • Regulatory protein SIR2 homolog 1
  • SIR2-like protein 1
  • SIR2alpha
  • Sir2
  • mSIR2a
  • SirtT1 75 kDa fragment
  • 75SirT1
  • Sirt1(Sir2), Alexa Fluor® 488 Conjugate
Background InformationNAD-dependent protein deacetylase sirtuin-1 (UniProt Q923E4; also known as Sir2, mSIRT2a, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID 93759) in murine species. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms. Sirtuins have been implicated in the regulation of aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) & aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity and cellular functions. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 & pS47 targeted by CaMKKβ.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sirt1 (Sir2) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1(Sir2) in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-131) is shown to be suitable also for Western blotting, immunoprecipitation, and ChIP applications.
Biological Information
ImmunogenGST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.
Epitopea.a. 1-131
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes Sirt1.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sirt1
  • Sir2l1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~110 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sirt1(Sir2) in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-131-AF488 04055977300437