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MABN41 Anti-Sodium channel Nav1.7 Antibody, clone N68/6

This Anti-Sodium channel Nav1.7 Antibody, clone N68/6 is validated for use in IH, WB for the detection of Sodium channel Nav1.7.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience, Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: NENA, PN1, hNE-Na, FEB3B, NE-NA, ETHA, SCN9A UniProt Number: Q15858
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MABN41
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, HIHC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN41
DescriptionAnti-Sodium channel Nav1.7 Antibody, clone N68/6
Alternate Names
  • sodium channel, voltage-gated, type IX, alpha subunit
  • Sodium channel protein type IX subunit alpha
  • Voltage-gated sodium channel subunit alpha Nav1.7
  • Peripheral sodium channel 1
  • sodium channel, voltage-gated, type IX, alpha polypeptide
  • Nav1.7
  • sodium channel protein type 9 subunit alpha
  • voltage-gated sodium channel alpha subunit Nav1.7
  • Neuroendocrine sodium channel
Background InformationVoltage-gated sodium channel subunit alpha Nav1.7 (Nav1.7) is a multi-pass membrane protein containing a single IQ domain that belongs to the sodium channel family. NAV1.7 localizes to the terminal ends of sensory neurons and is believed to have a role in inflammatory pain development and mechanisms. It serves to regulate excitable membrane voltage-dependent Na+ ion permeability. There is a strong probability that Nav1.7 is involved in the transmission of nociceptive information, a suggested involvement supported by its role in a variety of chronic pain neuropathies induced by damage to the peripheral nerves. Nav1.7 is predominantly observed in dorsal root ganglion, and sensory and sympathetic neurons. Lower levels of the protein have been observed in MTC cell line, C-cell carcinoma, and smooth muscle cells. Defects in Nav1.7 expression are causal to several diseases such as autosomal recessive congenital indifference pain, primary erythermalgia, and paroxysmal extreme pain disorder or PEPD.
References
Product Information
FormatPurified
Control
  • Rat cerebellum tissue
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sodium channel Nav1.7 Antibody, clone N68/6 is validated for use in IH, WB for the detection of Sodium channel Nav1.7.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern Blot Analysis: A previous lot was used by an independent laboratory in HEK293 cell lysate. (James Trimmer, UC Davis/NIH NeuroMab Facility, Department of Neurobiology, Physiology and Behavior, UC Davis, Davis CA 95616-8519. neuromab@ucdavis.edu.)
Biological Information
ImmunogenRecombinant protein corresponding to human Nav1.7.
CloneN68/6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq].
Gene Symbol
  • NENA
  • PN1
  • hNE-Na
  • FEB3B
  • NE-NA
  • ETHA
  • SCN9A
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain (By similarity).
SIZE: 1988 amino acids; 226342 Da

SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide. Interacts with NEDD4 and NEDD4L (By similarity).

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Note=In neurite terminals (By similarity).

TISSUE SPECIFICITY: Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system while isoform 2 is expressed preferentially in the dorsal root ganglion.

DOMAIN: The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

PTM: Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4 (By similarity).

DISEASE: Defects in SCN9A are the cause of primary erythermalgia [MIM:133020]. It is an autosomal dominant disease characterized by recurrent episodes of severe pain associated with redness and warmth in the feet or hands. & Defects in SCN9A are the cause of autosomal recessive congenital indifference to pain [MIM:243000]; also known as channelopathy-associated insensitivity to pain. Affected individuals have a congenital inability to perceive any form of pain, in any part of the body. All other sensory modalities are preserved and the peripheral and central nervous systems are apparently intact. Patients perceive the sensations of touch, warm and cold temperature, proprioception, tickle and pressure, but not painful stimuli. There is no evidence of a motor or sensory neuropathy, either axonal or demyelinating. & Defects in SCN9A are a cause of paroxysmal extreme pain disorder (PEPD) [MIM:167400]; previously known as familial rectal pain (FRP). PEPD is an autosomal dominant paroxysmal disorder of pain and autonomic dysfunction. The distinctive features are paroxysmal episodes of burning pain in the rectal, ocular, and mandibular areas accompanied by autonomic manifestations such as skin flushing.

SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Molecular WeightObserved at ~ 230 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in rat cerebellum tissue.

Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Nav1.7 in rat cerebellum tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN41 04053252553394

Documentation

Anti-Sodium channel Nav1.7 Antibody, clone N68/6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Sodium channel Nav1.7 Antibody, clone N68/6 Certificates of Analysis

TitleLot Number
Anti-Sodium channel Nav1.7, clone N68/6 - 2343902 2343902
Anti-Sodium channel Nav1.7, clone N68/6 - 2395804 2395804
Anti-Sodium channel Nav1.7, clone N68/6 - 2166826 2166826
Anti-Sodium channel Nav1.7, -2531013 2531013
Anti-Sodium channel Nav1.7, -2575139 2575139
Anti-Sodium channel Nav1.7, -2593147 2593147
Anti-Sodium channel Nav1.7, -2601668 2601668
Anti-Sodium channel Nav1.7, -2653107 2653107
Anti-Sodium channel Nav1.7, -2660171 2660171
Anti-Sodium channel Nav1.7, -2697605 2697605
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References

Reference overviewPub Med ID
Assembly and validation of versatile transcription activator-like effector libraries.
Li, Y; Ehrhardt, K; Zhang, MQ; Bleris, L
Scientific reports  4  4857  2014

Show Abstract 		24798576 24798576