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AB5535-AF488 Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate

This Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: SOX9 UniProt Number: P48436
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AB5535-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, M, ChICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue NumberAB5535-AF488
DescriptionAnti-Sox9 Antibody, Alexa Fluor® 488 conjugate
Alternate Names
  • Transcription factor SOX-9
  • Sox9
Background InformationTranscription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences that encode for the HMG-box domains that mediate sequence-specific DNA binding. Sox genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Sox9.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sox9 in HepG2 cells.
The unconjugated version (Cat. No. AB5535) is also shown to be suitable for Western blotting and immunohistochemistry applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Sox9.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Rat
  • Human
  • Mouse
  • Chicken
Species Reactivity NoteRat, Human, Mouse, Chicken. Predicted to react with Bovine, Feline, Horse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SOX9
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight55-65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sox9 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5535-AF488 04055977277173

Documentation

Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Sox9 Antibody, Alexa Fluor® 488 conjugate Certificates of Analysis

TitleLot Number
Anti-Sox9, Alexa Fluor® 488 conjugate 2895556
Anti-Sox9, Alexa Fluor® 488 conjugate - 3128128 3128128
Anti-Sox9, Alexa Fluor® 488 conjugate - 3229373 3229373
Anti-Sox9, Alexa Fluor® 488 conjugate - 3384386 3384386
Anti-Sox9, Alexa Fluor® 488 conjugate - 3727570 3727570
Anti-Sox9, Alexa Fluor® 488 conjugate - 3764361 3764361
Anti-Sox9, Alexa Fluor® 488 conjugate - 3888783 3888783
Anti-Sox9, Alexa Fluor® 488 conjugate - 3935881 3935881
Anti-Sox9, Alexa Fluor® 488 conjugate - 4077593 4077593
Anti-Sox9, Alexa Fluor® 488 conjugate -2860935 2860935
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.