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07-645-25UG Anti-Sp1 (rabbit polyclonal)

Use Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.

Anti-Sp1 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TSFP1 UniProt Number: P08047
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07-645-25UG
25 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, REMSA, IF, IP, WB, ChIPRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-645-25UG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Sp1 (rabbit polyclonal)
Alternate Names
  • Sp1 transcription factor
  • specificity protein 1
Background InformationSp1 is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 95 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Sp1 has always been described as a ubiquitous transcription factor that is required for the constitutive and inducible expression of a variety of genes, such as in cell cycle or mammalian development.
References
Product Information
FormatPurified
Control
  • HeLa nuclear extract.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationUse Anti-Sp1 Antibody (Rabbit Polyclonal Antibody) validated in ChIP, EMSA, IF, IP, WB to detect Sp1 also known as Sp1 transcription factor, specificity protein 1.
Key Applications
  • Electrophoretic Mobility Shift Assay
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunoprecipitation:
4 µg of this lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
NOTE: A previous lot successfully immunoprecipitated the α-1 subunit using the Catch and Release v2.0 Reversible Immunoprecipitation System (Catalog # 17-500). See manual for more information and protocol: www.millipore.com

Chromatin Immunoprecipitation:
Reported by an independent laboratory.

Immunofluorescence:
Reported by an independent laboratory.

Electrophoretic Mobility Shift Assay (EMSA):
1 µL of a previous lot of this antibody has been reported by an independent laboratory to supershift DNA complexes containing recombinant Sp1 and endogenous Sp1 from HeLa cells in EMSA.
Biological Information
ImmunogenFull length human Sp1 protein.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes Sp1, Mr 105 kDa and 95 kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman and mouse. Expected to cross react with rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TSFP1
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.
SIZE: 785 amino acids; 80693 Da
SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.
SUBCELLULAR LOCATION: Nucleus.
PTM: O-glycosylated; contains N-acetylglucosamine side chains.
SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers.
Molecular WeightVaries
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis:
1:500 dilution of this lot detected SP1 on 10 μg of HeLa lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-645-25UG 04054839337390