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04-772 Anti-Src Antibody, CT, clone NL19, rabbit monoclonal

Detect Src using this Anti-Src Antibody, CT, clone NL19, validated for use in western blotting & immunoprecipitation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: SRC, c-src, p60-Src, SRC1, c-SRC, ASV, pp60c-src UniProt Number: P12931
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04-772
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIP, WBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-772
Replaces05-772
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Src Antibody, CT, clone NL19, rabbit monoclonal
Alternate Names
  • Proto-oncogene tyrosine-protein kinase SRC
  • Protooncogene SRC
  • Rous sarcoma tyrosine kinase pp60c-src
  • Tyrosine-protein kinase SRC-1
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Background InformationSRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
References
Product Information
FormatCulture Supernatant
Control
  • RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
PresentationCultured supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationDetect Src using this Anti-Src Antibody, CT, clone NL19, validated for use in western blotting & immunoprecipitation.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.
Biological Information
ImmunogenKLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
CloneNL19
HostRabbit
SpecificitySrc-tyrosine kinase family
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NotePredicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • SRC
  • c-src
  • p60-Src
  • SRC1
  • c-SRC
  • ASV
  • pp60c-src
UniProt Number
UniProt SummarySIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight~53-62kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-772 04053252402517

Documentation

Anti-Src Antibody, CT, clone NL19, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-Src Antibody, CT, clone NL19, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-Src, CT, clone NL19 - 2444788 2444788
Anti-Src, CT, clone NL19 - 2492805 2492805
Anti-Src, CT, clone NL19 - 2233813 2233813
Anti-Src, CT, clone NL19 - 3267895 3267895
Anti-Src, CT, clone NL19 - 3524300 3524300
Anti-Src, CT, clone NL19 - DAM1561692 DAM1561692
Anti-Src, CT, clone NL19 -2664239 2664239

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies