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MAB5200 Anti-Synaptotagmin I Antibody

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MAB5200
200 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, Ch, M, R, Rb, Fg, ShkIP, WB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5200
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Synaptotagmin I Antibody
Alternate Names
  • p65
  • Syt1
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in PBS with 50% glycerol and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Synaptotagmin I Antibody is validated for use in IP, WB, IC for the detection of Synaptotagmin I.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern blot: 5 μg/mL (colormetric) Immunocytochemistry

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Biological Information
ImmunogenRat brain synaptotagmin.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySynaptotagmin 1, also known as p65. By Western blot MAB5200 reacts with a single protein with a molecular weight of approximately 65 kDa. MAB5200 has not been epitope mapped.
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Chicken
  • Mouse
  • Rat
  • Rabbit
  • Frog
  • Shark
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
Gene Symbol
  • SYT1
  • DKFZp781D2042
  • SVP65
  • SytI
  • SYT
  • p65
  • P65
  • Synaptotagmin-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P21579 # May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains (By similarity).
SIZE: 422 amino acids; 47573 Da
SUBUNIT: Homotetramer (Probable). Interacts with STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity).
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note=Synaptic vesicles and chromaffin granules.
DOMAIN: SwissProt: P21579 The first C2 domain mediates Ca(2+)-dependent phospholipid binding. & The second C2 domain mediates interaction with SV2A and STN2 (By similarity).
SIMILARITY: Belongs to the synaptotagmin family. & Contains 2 C2 domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 12 months.Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB5200 04053252726040

Documentation

Anti-Synaptotagmin I Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Synaptotagmin I Antibody Certificates of Analysis

TitleLot Number
MOUSE ANTI-SYNAPTOTAGMIN 2483186
MOUSE ANTI-SYNAPTOTAGMIN MONOCLONAL ANTIBODY - 2390418 2390418
MOUSE ANTI-SYNAPTOTAGMIN - 2522224 2522224
MOUSE ANTI-SYNAPTOTAGMIN - 3182124 3182124
MOUSE ANTI-SYNAPTOTAGMIN - 3209771 3209771
MOUSE ANTI-SYNAPTOTAGMIN - 3552756 3552756
MOUSE ANTI-SYNAPTOTAGMIN - 3776749 3776749
MOUSE ANTI-SYNAPTOTAGMIN - 3863412 3863412
MOUSE ANTI-SYNAPTOTAGMIN -2519247 2519247
MOUSE ANTI-SYNAPTOTAGMIN -2689160 2689160

References

Reference overviewPub Med ID
Presynaptic GABA(B) receptors decrease neurotransmitter release in vestibular nuclei neurons during vestibular compensation.
Shao, M; Reddaway, R; Hirsch, JC; Peusner, KD
Neuroscience  223  333-54  2012

Show Abstract
22871524 22871524
Adaptation of chicken vestibular nucleus neurons to unilateral vestibular ganglionectomy.
M Shao,A Popratiloff,J Yi,A Lerner,J C Hirsch,K D Peusner
Neuroscience  161  2009

Show Abstract
19375485 19375485
AMPA receptor subunit expression in chick vestibular nucleus neurons.
Anastas Popratiloff, Ya-Xian Wang, Jared Narvid, Ronald S Petralia, Christian Giaume, Kenna D Peusner
Journal of neuroscience research  76  662-77  2004

Show Abstract
15139025 15139025
Synaptotagmins in membrane traffic: which vesicles do the tagmins tag?
Marqueze, B, et al
Biochimie, 82:409-20 (2000)  2000

10865128 10865128
Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue
Matthew, W D, et al
J Cell Biol, 91:257-69 (1981)  1981

7298720 7298720

Data Sheet

Title
MOUSE ANTI-SYNAPTOTAGMIN MONOCLONAL ANTIBODY