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MABE462 Anti-TET2 Antibody, clone hT2H 21F11

Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.

Anti-TET2 Antibody, clone hT2H 21F11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: TET2 UniProt Number: Q6N021
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MABE462
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MChIP, WB, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE462
DescriptionAnti-TET2 Antibody, clone hT2H 21F11
Alternate Names
  • Methylcytosine dioxygenase TET2
Background InformationMethylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).
References
Product Information
FormatPurified
Control
  • MOLT-4 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate.
Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.
Biological Information
ImmunogenRecombinant protein corresponding to the N-terminus of human TET2.
EpitopeN-terminus
ClonehT2H21F11
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes the N-terminus of TET2
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TET2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE462 04053252911767

Documentation

Anti-TET2 Antibody, clone hT2H 21F11 SDS

Title

Safety Data Sheet (SDS) 

Anti-TET2 Antibody, clone hT2H 21F11 Certificates of Analysis

TitleLot Number
Anti-TET2, clone hT2H 21F11 2480977
Anti-TET2, clone hT2H 21F11 - 3218253 3218253
Anti-TET2, clone hT2H 21F11 - 3305921 3305921
Anti-TET2, clone hT2H 21F11 - 3365618 3365618
Anti-TET2, clone hT2H 21F11 - 3560115 3560115
Anti-TET2, clone hT2H 21F11 - 3739776 3739776
Anti-TET2, clone hT2H 21F11 - 3933214 3933214
Anti-TET2, clone hT2H 21F11 - 3933647 3933647
Anti-TET2, clone hT2H 21F11 - 4194302 4194302
Anti-TET2, clone hT2H 21F11 - Q2201628 Q2201628
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References

Reference overviewApplicationPub Med ID
PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation.
de la Rica, Lorenzo, et al.
Genome Biol., 14: R99 (2013)  2013

Show Abstract 		Chromatin Immunoprecipitation (ChIP)24028770 24028770

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics