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ABD125 Anti-TFE3 Antibody

This Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3.

Anti-TFE3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: TFE3 UniProt Number: P19532
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ABD125
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD125
DescriptionAnti-TFE3 Antibody
Alternate Names
  • TFE3
  • Transcription factor E3
  • Class E basic helix-loop-helix protein 33
  • bHLHe33
Background InformationThe DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFE3 is ubiquitously expressed and can directly associates with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo but at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization of the placenta. Recently, Smith et al. have proven that the exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor TFE3 (Cell 153, 335–347, April 11, 2013).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationThis Anti-TFE3 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of TFE3.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A representative lot was used by an independent laboratory on TFE3 overexpressing cell lines, epiblast and primitive endodermal cells (Smith, A., et al. (2013). Cell. 153, 335–347).
Biological Information
Immunogenlinear peptide corresponding to Human TFE3.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TFE3
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~ 65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in a HEK293T cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected TFE3 in 10 µg of HEK293T cell lysate.
Uncharacterized band(s) may appear in some lysates with longer exposure times.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABD125 04055977039450