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MABS2053-25UG Anti-TFF3 Antibody, clone TFF-286

Anti-TFF3, clone TFF-286, Cat. No. MABS2053, is a mouse monoclonal antibody that detects Trefoil factor 3 (TFF3) and has been tested for use in ELISA and Western Blotting.

MABS2053-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2053-25UG
DescriptionAnti-TFF3 Antibody, clone TFF-286
Alternate Names
  • Trefoil factor 3
  • Intestinal trefoil factor
  • hITF
  • Polypeptide P1.B
  • hP1.B
Background InformationTrefoil factor 3 (UniProt: Q07654; also known as TFF3, Intestinal trefoil factor, hITF, Polypeptide P1.B, hP1.B) is encoded by the TFF3 (also known as ITF, TFI) gene (Gene ID: 7033) in human. TFF3 is a small soluble peptide secreted by mucin-producing intestinal epithelial cells and goblet cells. It is also expressed in the uterus, breast, and in some parts of the hypothalamus and pituitary gland. TFF3 is synthesized with a signal peptide (aa 1-35), which is subsequently cleaved off to produce the mature form. It can exist as a monomer or disulfide-linked homodimer. Upon dimerization, the additional 3(10)-helix involving residues 53-55 occur in the TFF3 homodimer that contributes to its distinct structure. The monomeric form is shown to induce cell migration by activating the MAPK pathway independently of the epidermal growth factor and the homodimeric form is shown to have an anti-apoptotic effect on epithelial cells. TFF3 is reported to promote mucosal wound healing in mice and humans and its elevated expression has been linked with gastric adenocarcinoma progression and biliary diseases. TFF3 expression in the intestine is shown to be regulated by food intake and its overexpression is shown to improve glucose tolerance by blocking the expression of gluconeogenic enzymes. TFF3 treatment in animals is reported to reduce fasting insulin levels. (Ref.: Xue, Y., et al. (2013). PLoS One. 8(9); e75240; Ge, H., et al. (2015). PLoS One. 10(6); e0126924; Khummuang, S., et al. (2017). Biological Procedures Online (2017) 19;14).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-TFF3, clone TFF-286, Cat. No. MABS2053, is a mouse monoclonal antibody that detects Trefoil factor 3 (TFF3) and has been tested for use in ELISA and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
Application NotesELISA Analysis: A representative lot detected TFF3 in ELISA applications (Khummuang. S., et. al. (2017). Biol Proced Online. 19:14).

Western Blotting Analysis: A representative lot detected TFF3 in Western Blotting applications (Khummuang. S., et. al. (2017). Biol Proced Online. 19:14).
Biological Information
ImmunogenRecombinant human dimeric TFF3.
CloneTFF-286
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone TFF-286 is a mouse monoclonal antibody that detects human Trefoil factor 3 (TFF3).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TFF3
  • ITF
  • TFI
Purification MethodProtein G purified
UniProt Number
Molecular Weight~26 kDa observed for His-and thioredoxin tagged recombinant human TFF3; 10.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in recombinant Human Trefoil Factor-3, expressed in E. coli.

Western Blotting Analysis: 2 µg/mL of this antibody detected His-and Thioredoxin tagged recombinant human TFF3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS2053-25UG 04061841134824

Documentation

Anti-TFF3 Antibody, clone TFF-286 SDS

Title

Safety Data Sheet (SDS) 

Anti-TFF3 Antibody, clone TFF-286 Certificates of Analysis

TitleLot Number
Anti-TFF3, clone TFF-286 - 3327733 3327733

References

Reference overviewPub Med ID
Production of Monoclonal Antibodies against Human Trefoil Factor 3 and Development of a Modified-Sandwich ELISA for Detection of Trefoil Factor 3 Homodimer in Saliva.
Khummuang, S; Phanphrom, W; Laopajon, W; Kasinrerk, W; Chaiyarit, P; Pata, S
Biol Proced Online  19  14  2017

Show Abstract 		29151819 29151819