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07-1527 Anti-TFIIF-alpha Antibody

Use Anti-TFIIF-alpha Antibody (rabbit polyclonal antibody) validated in WB, IP to detect TFIIF-alpha also known as Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74.

Anti-TFIIF-alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: GTF2F1, RAP74 UniProt Number: P35269
07-1527
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, B, Po, ChpWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1527
DescriptionAnti-TFIIF-alpha Antibody
Alternate Names
  • General transcription factor IIF subunit 1
  • General transcription factor IIF 74 kDa subunit
  • Transcription initiation factor IIF subunit alpha
  • TFIIF-alpha
  • Transcription initiation factor RAP74
Background InformationTFIIF, a general transcription initiation factor, is a heterodimer composed of 74 kDa, GTF2F1 (Rap74), and a 30 kDa (GTF2F2) subunits. TFIIF is involved in transcription initiation and elongation, and the CTD of GTF2F1 binds to the RNA polymerase II enzyme, the general transcription factor TFIIB, and a CTD phosphate, FCP1. Studies point to TFIIF involvement in both early and late transcriptional stages. Data also suggest that GTF2F1 is involved in the in the recycling of the RNA Polymerase II via FCP1's CTD-specific phosphatase activity.
References
Product Information
FormatAffinity Purified
Control
  • K562 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationUse Anti-TFIIF-alpha Antibody (rabbit polyclonal antibody) validated in WB, IP to detect TFIIF-alpha also known as Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated TFIIF-alpha from K562 RIPA lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human TFIIF-alpha at the C-terminus.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes GTF2F1 at the C-terminus.
Species Reactivity
  • Human
  • Bovine
  • Pig
  • Chimpanzee
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Porcine, Orangutan, and Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GTF2F1
  • RAP74
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2 and CTDP1. Interacts with TAF6/TAFII80.

SUBCELLULAR LOCATION: Nucleus.

INDUCTION: Up-regulated in response to enterovirus 71 (EV71) infection.

PTM: Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.

SEQUENCE SIMILARITIES: Belongs to the TFIIF alpha subunit family.

CAUTION: Was reported (Ref.9) to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.
Molecular Weight~74 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected TFIIF-alpha on 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1527 04053252421136