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AB6000 Anti-TIMP-3 Antibody, CT

This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.

Anti-TIMP-3 Antibody, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: MMPs & TIMPs Gene Symbol: TIMP3, K222, TIMP-3, SFD UniProt Number: P35625
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AB6000
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, Po, Ca, Mk, Pm, Eq, BWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB6000
ReplacesAB802
DescriptionAnti-TIMP-3 Antibody, CT
Alternate Names
  • MIG-5
  • TIMP metallopeptidase inhibitor 3
  • Tissue inhibitor of metalloproteinases 3
Background InformationTissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.
References
Product Information
FormatAffinity Purified
Control
  • HL-60 cell lysate in PMA-conditioned media.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThe antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.
Species Reactivity
  • Human
  • Rat
  • Pig
  • Canine
  • Monkey
  • Primate
  • Horse
  • Bovine
Species Reactivity NoteHuman and rat. Predicted to react with pig, canine, monkey, primate, equine, and bovine based on 100% sequence homology and mouse at 95% homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq]
Gene Symbol
  • TIMP3
  • K222
  • TIMP-3
  • SFD
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. May form part of a tissue- specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15.

SIZE: 211 amino acids; 24145 Da

SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.

DISEASE: Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years.

SIMILARITY: Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
Molecular WeightBands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB6000 04053252279706

Documentation

Anti-TIMP-3 Antibody, CT SDS

Title

Safety Data Sheet (SDS) 

Anti-TIMP-3 Antibody, CT Certificates of Analysis

TitleLot Number
Anti-TIMP-3, C-terminus 2470959
Anti-TIMP-3, C-terminus - 2145977 2145977
Anti-TIMP-3, C-terminus - 2370189 2370189
Anti-TIMP-3, C-terminus - NG1756936 NG1756936
Anti-TIMP-3, C-terminus - 1983711 1983711
Anti-TIMP-3, C-terminus - 2023685 2023685
Anti-TIMP-3, C-terminus - 2246836 2246836
Anti-TIMP-3, C-terminus - 3182121 3182121
Anti-TIMP-3, C-terminus - 3288514 3288514
Anti-TIMP-3, C-terminus - 3365625 3365625
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