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MABS1719 Anti-TMEM64 Antibody, clone 7A2.1

Detect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.

Anti-TMEM64 Antibody, clone 7A2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: TMEM64 UniProt Number: Q6YI46
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MABS1719
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1719
DescriptionAnti-TMEM64 Antibody, clone 7A2.1
Alternate Names
  • Transmembrane protein 64
Background InformationTransmembrane protein 64 (UniProt Q6YI46) is encoded by the TMEM64 gene (Gene ID 169200) in human. TMEM64 is a 6-transmembrane protein that plays a role in modulating Wnt/ -catenin signaling-mediated osteoblast and adipocyte differentiation. TMEM64 interacts and positively regulates sarcoplasmic endoplasmic reticulum Ca(2+) ATPase 2 (SERCA2) activity, which is important for RANKL-induced Ca(2+) oscillation, Ca(2+)/calmodulin-dependent protein kinases (CaMK) IV activity and mitochondrial ROS formation needed for for osteoclast formation. Mice with Tmem64 deficiency are characterized by a high-bone-mass phenotype, displaying higher levels of ALP, osteocalcin (Ocn), and type 1 collagen (Col 1a) transcripts than in wild-type mice. Tmem64-knockout in mice is reported to markedly enhance osteoblast differentiation and inhibit adipocyte differentiation from bone marrow-derived stromal cells (BMSCs), resulting in a preferential switch of mesenchymal progenitors favoring the osteogenic over the adipogenic lineage.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect TMEM64 using this mouse monoclonal Anti-TMEM64, clone 7A2.1 Antibody, Cat. No. MABS1719, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of SK-MEL-28 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminal end sequence of human and rat TMEM64.
Clone7A2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThe immunogen sequence is 100% conserved between human and rat species and present in all four human spliced isoforms reported by UniProt (Q6YI46 & D3ZV19). Murine cross-reactivity is highly likely, but has not been confirmed.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TMEM64
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~45 kDa observed. 39.67/12.95/14.25/33.97 kDa (human isoform 1/2/3/4) and 39.53 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TMEM64 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS1719 04054839081422