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MABN1806 Anti-TPP1 Antibody, clone 2E12

Anti-TPP1, clone 2E12, Cat. No. MABN1806, is a highly specific mouse monoclonal antibody, that targets TPP1/Tripeptidyl-peptidase 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TPP1 Antibody, clone 2E12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TPP1, CLN2, GIG1, UNQ267/PRO304 UniProt Number: O14773
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MABN1806
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1806
DescriptionAnti-TPP1 Antibody, clone 2E12
Alternate Names
  • Tripeptidyl-peptidase 1
  • Cell growth-inhibiting gene 1 protein
  • Lysosomal pepstatin-insensitive protease
  • LPIC
  • Tripeptidyl aminopeptidase
Background InformationTripeptidyl-peptidase 1 (UniProt: O14773; also known as TPP1, Cell growth-inhibiting gene 1 protein, Lysosomal pepstatin-insensitive protease, LPIC, Tripeptidyl aminopeptidase) is encoded by the TPP1 (also known as CLN2, GIG1, UNQ267/PRO304) gene (Gene ID: 1200) in human. TPP1 is a lysosomal serine protease that acts as a non-specific peptidase and sequentially removes tripeptides from the free N termini of small polypeptides produced by lysosomal proteinases. TPP1 is expressed in several tissues, with tissues associated with peptide hormone production displaying higher level of expression. Human TPP I is synthesized as a zymogen with an apparent mass of 68 kDa, which is processed to a 48 kDa mature enzyme by removal of 179 amino acid prodomain. TPP I zymogen has five potential N-glycosylation sites at amino acid positions 210, 222, 286, 313, and 443. The N-linked glycans can add around 10 kDa to the molecular mass of TPP 1. Like many other lysosomal hydrolases, TPP I proenzyme is able to autoactivate in the acidic pH in vitro. Mutations in TPP1 gene are shown to be a causative factor in ceroid lipofuscinosis, neuronal, 2 (CLN2) a progressive neurodegenerative, lysosomal storage disease, characterized by intracellular accumulation of autofluorescent liposomal material and clinically manifested seizures, dementia, visual loss, and/or cerebral atrophy.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TPP1, clone 2E12, Cat. No. MABN1806, is a highly specific mouse monoclonal antibody, that targets TPP1/Tripeptidyl-peptidase 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected TPP1 in human heart and human pancreas tissue.

Western Blotting Analysis: A 1:50 dilution from a representative lot detected TPP1 in CHO cells transfected with TPP1 and TPP1 mutant (Courtesy of Adam Golabek, Ph.D., New York State Institute for Basic Research in Developmental Disabilities, New York, USA).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TPP1 in CHO cells expressing WT TPP1 (Courtesy of Adam Golabek, Ph.D., New York State Institute for Basic Research in Developmental Disabilities, New York, USA).
Biological Information
ImmunogenA recombinant fragment corresponding to amino acids 195-563 of human TPP1.
Clone2E12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2E12 specifically immunostains tripeptidyl peptidase 1 in human placenta, heart, and pancreas.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TPP1
  • CLN2
  • GIG1
  • UNQ267/PRO304
Purification MethodProtein G purified
UniProt Number
Molecular Weight61.25 kDa calculated (composed of 6 identical subunits)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human placenta tissue.

Immunohistochemistry Analysis: A 1:5,000 dilution of this antibody detected TPP1 in human placenta tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN1806 04054839168680