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MAB4360 Anti-TRA-1-60 Antibody, clone TRA-1-60

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MAB4360
100 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FC, IF, IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4360
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TRA-1-60 Antibody, clone TRA-1-60
Background InformationHuman embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
PresentationPurified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunofluorescence: A previous lot was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman embryonal carcinoma cell line 2102Ep
Cloneclone TRA-1-60
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
IsotypeIgM
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • TRA-1-60
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight235/410 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB4360 04053252610073

Documentation

Anti-TRA-1-60 Antibody, clone TRA-1-60 SDS

Title

Safety Data Sheet (SDS) 

Anti-TRA-1-60 Antibody, clone TRA-1-60 Certificates of Analysis

TitleLot Number
Anti-TRA-1-60, Clone TRA-1-60 - 4101851 4101851
Anti-TRA-1-60, Clone TRA-1-60 - 4196044 4196044
Anti-TRA-1-60, clone TRA-1-60 2462881
Anti-TRA-1-60, clone TRA-1-60 - 2140039 2140039
Anti-TRA-1-60, clone TRA-1-60 - 2366550 2366550
Anti-TRA-1-60, clone TRA-1-60 - 2430441 2430441
Anti-TRA-1-60, clone TRA-1-60 - 2016625 2016625
Anti-TRA-1-60, clone TRA-1-60 - 2271178 2271178
Anti-TRA-1-60, clone TRA-1-60 - 3257043 3257043
Anti-TRA-1-60, clone TRA-1-60 - 3436989 3436989

References

Reference overviewApplicationSpeciesPub Med ID
Impaired respiratory function in MELAS-induced pluripotent stem cells with high heteroplasmy levels.
Kodaira, M; Hatakeyama, H; Yuasa, S; Seki, T; Egashira, T; Tohyama, S; Kuroda, Y; Tanaka, A; Okata, S; Hashimoto, H; Kusumoto, D; Kunitomi, A; Takei, M; Kashimura, S; Suzuki, T; Yozu, G; Shimojima, M; Motoda, C; Hayashiji, N; Saito, Y; Goto, Y; Fukuda, K
FEBS open bio  5  219-25  2015

Show Abstract
25853038 25853038
An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.
Nishishita, N; Muramatsu, M; Kawamata, S
American journal of stem cells  4  38-49  2015

Show Abstract
25973330 25973330
A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach.
Chou, BK; Gu, H; Gao, Y; Dowey, SN; Wang, Y; Shi, J; Li, Y; Ye, Z; Cheng, T; Cheng, L
Stem cells translational medicine  4  320-32  2015

Show Abstract
25742692 25742692
A method for human teratogen detection by geometrically confined cell differentiation and migration.
Xing, J; Toh, YC; Xu, S; Yu, H
Scientific reports  5  10038  2015

Show Abstract
25966467 25966467
Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
Cerbini, T; Funahashi, R; Luo, Y; Liu, C; Park, K; Rao, M; Malik, N; Zou, J
PloS one  10  e0116032  2015

Show Abstract
25587899 25587899
Derivation of induced pluripotent stem cells from orangutan skin fibroblasts.
Ramaswamy, K; Yik, WY; Wang, XM; Oliphant, EN; Lu, W; Shibata, D; Ryder, OA; Hacia, JG
BMC research notes  8  577  2015

Show Abstract
26475477 26475477
Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
Tolar, J; McGrath, JA; Xia, L; Riddle, MJ; Lees, CJ; Eide, C; Keene, DR; Liu, L; Osborn, MJ; Lund, TC; Blazar, BR; Wagner, JE
The Journal of investigative dermatology  134  1246-54  2014

Show Abstract
24317394 24317394
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada, Mitsutoshi, et al.
Nature, (2014)  2014

Show Abstract
24776804 24776804
Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions.
Kishino, Y; Seki, T; Fujita, J; Yuasa, S; Tohyama, S; Kunitomi, A; Tabei, R; Nakajima, K; Okada, M; Hirano, A; Kanazawa, H; Fukuda, K
PloS one  9  e97397  2014

Show Abstract
24824994 24824994
Development of a high-yield technique to isolate spermatogonial stem cells from porcine testes.
Park, MH; Park, JE; Kim, MS; Lee, KY; Park, HJ; Yun, JI; Choi, JH; Lee, Es; Lee, ST
Journal of assisted reproduction and genetics  31  983-91  2014

Show Abstract
Fluorescence Activated Cell Sorting (FACS), Immunocytochemistry24938360 24938360

Technical Info

Title
A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium
Millipore Tools for Characterizing Induced Pluripotent Stem Cells
Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

Posters

Title
Poster: A Novel Serum-Free and Animal Component-Free Culture Medium for the Propagation and Maintenance of Human Embryonic Stem Cells