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MABE432 Anti-TRIM33 Antibody, clone 3B5.1

This Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.

Anti-TRIM33 Antibody, clone 3B5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: TRIM33, KIAA1113, RFG7, TIF1G UniProt Number: Q9UPN9
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MABE432
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE432
DescriptionAnti-TRIM33 Antibody, clone 3B5.1
Alternate Names
  • E3 ubiquitin-protein ligase TRIM33
  • Ectodermin homolog
  • RET-fused gene 7 protein
  • Protein Rfg7
  • Transcription intermediary factor 1-gamma
  • TIF1-gamma
  • Tripartite motif-containing protein 33
Background InformationTRIM33, also known as E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, or Tripartite motif-containing protein 33, and encoded by the gene TRIM33/KIAA1113/RFG7/TIF1G, is an E3 ubiquitin protein ligase. Among its targets is SMAD4 for which ubiquitination facilitates its nuclear exclusion and degradation. SMAD3 is also a target for ubiquitination, and together, TRIM33's association promotes erythroid differentiation of hematopoietic stem cells. Finally mono-ubiquitination of various targets by TRIM33 affects their ability to form complexes which often hampers their activity and thus regulates their subsequent actions. Such is the case of SMAD4 and its interaction with TGF-beta/BMP signaling. Monoubiquitination of SMAD4 by TRIM33 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. TRIM33 is localized in nucleus in nuclear dots or bodies and is expressed in most tissues and stem cells during development as well as in the adult in multiple tissues.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-TRIM33 Antibody, clone 3B5.1 is validated for use in Western Blotting and Immunocytochemistry for the detection of TRIM33.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TRIM33 in H9 stem cells.
Alexa Fluor® is a registered trademark of Life Technologies®.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human TRIM33.
Clone3B5.1
Concentration1 mg/mL
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TRIM33
  • KIAA1113
  • RFG7
  • TIF1G
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~123 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TRIM33 in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE432 04053252978777