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610205 Anti-Tie2 Mouse mAb (Ab33)

This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.

Anti-Tie2 Mouse mAb (Ab33) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiopoietin Receptor, Anti-Tek

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610205
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, Porcine, RMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
610205-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes the ~140-45 kDa Tie2 protein.
Catalogue Number610205
Brand Family Calbiochem®
SynonymsAnti-Angiopoietin Receptor, Anti-Tek
References
ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
Wong, A.L., et al. 1997. Circ. Res. 81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Not Paraffin Sections
Application NotesFrozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
Application CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
ImmunogenHuman
CloneAb33
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Porcine
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
610205-50UG 04055977264197

Documentation

Anti-Tie2 Mouse mAb (Ab33) SDS

Title

Safety Data Sheet (SDS) 

Anti-Tie2 Mouse mAb (Ab33) Certificates of Analysis

TitleLot Number
610205

References

Reference overview
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
Wong, A.L., et al. 1997. Circ. Res. 81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-November-2017 JSW
SynonymsAnti-Angiopoietin Receptor, Anti-Tek
ApplicationFrozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
BackgroundAngiopoietin-1 and its naturally occurring inhibitor, angiopoietin-2, exert their effects by inducing autophosphorylation of their signaling receptor Tie2. Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr1100) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag® sequence
CloneAb33
IsotypeIgG₁
Specieshuman, mouse, porcine, rat
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsFor frozen sections, tissues were treated with Triton®-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLin, P., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 8829.
Wong, A.L., et al. 1997. Circ. Res. 81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer 77, 51.