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650297 Anti-Trypsin Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
650297-1ML Plastic ampoule 1 ml
Description
OverviewRecognizes cationic trypsin in pancreas tissue. Does not cross-react with other plasma proteins.
Catalogue Number650297
Brand Family Calbiochem®
Application Data
Detection of trypsin by immunoblotting. Samples: Chymotrypsin (lane 1), pancreatic extract (lane 2), trypsin (0.15 µg) (lane 3), trypsin (0.3 µg) (lane 4), trypsin (0.6 µg) (lane 5), and trypsin (1.2 µg) (lane 6), with low molecular weight marker (lane 7). Primary antibody: Anti-Trypsin Rabbit pAb (Cat. No. 650297) (1:4000). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.4.
Positive controlHuman pancreas tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoelectrophoresis
Application NotesELISA (≥1:250)
Immunoblotting (1:2000)
Immunoelectrophoresis (see comments)
Application CommentsDoes not cross-react with other plasma proteins. Monospecific as determined by immunoelectrophoresis against human pancreatic extract and against human trypsin. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman pancreatic trypsin
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
650297-1ML 04055977185232

Documentation

Anti-Trypsin Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Trypsin Rabbit pAb Certificates of Analysis

TitleLot Number
650297
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2007 RFH
ApplicationELISA (≥1:250)
Immunoblotting (1:2000)
Immunoelectrophoresis (see comments)
Application Data
Detection of trypsin by immunoblotting. Samples: Chymotrypsin (lane 1), pancreatic extract (lane 2), trypsin (0.15 µg) (lane 3), trypsin (0.3 µg) (lane 4), trypsin (0.6 µg) (lane 5), and trypsin (1.2 µg) (lane 6), with low molecular weight marker (lane 7). Primary antibody: Anti-Trypsin Rabbit pAb (Cat. No. 650297) (1:4000). Detection: chemiluminescence.
DescriptionRabbit polyclonal antibody was purified by ammonium sulfate precipitation followed by DEAE-cellulose ion exchange chromatography. Recognizes cationic trypsin.
HostRabbit
Immunogen speciesHuman
Immunogenhuman pancreatic trypsin
IsotypeIgG
Specieshuman
Positive controlHuman pancreas tissue
FormLiquid
FormulationIn PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with other plasma proteins. Monospecific as determined by immunoelectrophoresis against human pancreatic extract and against human trypsin. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling