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MABT1332-25UG Anti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8

Anti-Tspan-8, clone 40/15-1B11-63-76-8 (Anti-Tspan8-A), Cat. No. MABT1332, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABT1332-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABT1332-25UG
DescriptionAnti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8
Alternate Names
  • Tetraspanin-8
  • Tspan-8
Background InformationTetraspanin-8 (UniProt: Q8R3G9; also known as Tspan8, Tspan-8) is encoded by the Tspan8 gene (Gene ID: 216350) in murine species. Tspan-8 is a multi-pass membrane protein of the tetraspanin family that is involved in the regulation of cell activation, growth, and motility. Members of this family have emerged as important regulators of expression levels, trafficking or post-translational modification of laminin-binding integrins. Tetraspanins are shown to associate with one another and cluster dynamically with a large variety of transmembrane and signal-transducing partners, forming specialized membrane microdomains called tetraspanin web. Tspan-8 is reported to be overexpressed during the progression of colorectal, liver, pancreatic and gastric cancers and its increased expression is shown to promote liver and lung metastasis. Overexpression of Tspan-8 has also been correlated with poor prognosis in human colorectal cancer. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tspan-8 and Tspan CD151 with integrin alpha6beta 4 and subsequent internalization of this integrin-tetraspanin complex, resulting in decreased cell-matrix adhesion and increased cell migration. Tspan-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. (Ref.: Kharbili, ME., et al. (2017). Oncotarget. 8(10); 17140-17155).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibod yIgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Tspan-8, clone 40/15-1B11-63-76-8 (Anti-Tspan8-A), Cat. No. MABT1332, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected Tspan-8 in Immunohistochemistry applications (Fu, N.Y., et. al. (2017). Nat Cell Biol. 19(3):164-176).

Western Blotting Analysis: 4 µg/mL from a representative lot detected Tspan-8 in LIM1215 positive for Tspan8 and HEK293T cell lysates.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the extracellular domain of murine Tetraspanin-8.
Epitopeextracellular domain
Clone40/15-1B11-63-76-8
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 40/15-1B11-63-76-8 is a rat monoclonal antibody that detects Tetraspanin-8. It targets an epitope with in the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Monkey based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tspan8
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.58 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Tspan-8 in mouse colon tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1332-25UG 04054839755262

Documentation

Anti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8 Certificates of Analysis

TitleLot Number
Anti-Tspan-8, clone 40/15-1B11-63-76-8 - 3208234 3208234

References

Reference overviewPub Med ID
Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive.
Fu, NY; Rios, AC; Pal, B; Law, CW; Jamieson, P; Liu, R; Vaillant, F; Jackling, F; Liu, KH; Smyth, GK; Lindeman, GJ; Ritchie, ME; Visvader, JE
Nat Cell Biol  19  164-176  2017

Show Abstract 		28192422 28192422