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ST1200 Anti-Ubiquitin Mouse mAb (FK2)

This Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin.

Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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ST1200
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
ST1200-100UG Glass bottle 100 μg
Description
OverviewRecognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins, but not free ubiquitin, in Raji cells.
Catalogue NumberST1200
Brand Family Calbiochem®
Application Data
Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
References
ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Application CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenunpurified polyubiquitinylated lysozyme
Immunogenhuman
CloneFK2
HostMouse
IsotypeIgG₁
SourceRaji cells
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ST1200-100UG 04055977224078

Documentation

Anti-Ubiquitin Mouse mAb (FK2) SDS

Title

Safety Data Sheet (SDS) 

Anti-Ubiquitin Mouse mAb (FK2) Certificates of Analysis

TitleLot Number
ST1200

References

Reference overview
Matsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-March-2009 JSW
ApplicationELISA (see application references)
Immunoblotting (1:1000)
Immunoprecipitation (see application references and comments)
Immunofluorescence (see application references)
Application Data
Detection of ubiquitin by immunoblotting. Samples: K48-linked ubiquitin chains (lanes A and D), K29-linked ubiquitin chains (lanes B and E), and K63-linked ubiquitin chains (lanes C and F). Primary antibodies: Anti-Ubiquitin (recognizing only polyubiquitinylated proteins) (1:1000) (lanes A-C) and Anti-Ubiquitin Mouse mAb (FK2) (Cat. No. ST1200) (1:1000) (lanes D-F). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K29-, K48-, and K63-linked polyubiquitinylated and monoubiquitinylated proteins.
HostMouse
Immunogen specieshuman
Immunogenunpurified polyubiquitinylated lysozyme
CloneFK2
IsotypeIgG₁
Speciesa broad range of species
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
SourceRaji cells
Preservative≤0.1% sodium azide
CommentsDoes not recognize free ubiquitin. For immunoprecipitation, once the antibody is immobilized, it demonstrates affinity for free ubiquitin as well as for poly- and monoubiquitinylated proteins. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Store diluted antibody at 4°C for up to 1 month.
Toxicity Standard Handling
ReferencesMatsumoto, M., et al. 2005. Proteomics 5, 4545.
Boname, J.N., et al. 2001. Immunity 15, 627.
Campbell, V., et al. 2001. Neuron 32, 1013.
Fujimuro, M., et al. 2003. FEBS Letts. 349, 173.
Application referencesELISA Takada, K., et al. 1995. Eur. J. Biochem. 233, 42. Takada, K., et al. 1997. Clin. chem. 43, 1188. Immunoprecipitation Takada, K., et al. 2001. Biochem. J. 356, 199. Boname, J.N. and Stevenson, P.G. 2001. Immunity 15, 627. Matsumoto, M., et al. 2005. Proteomics 5, 4145. Immunofluorescence Campbell, D.S. and Holt, C.E. 2001. Neuron 32, 1013.