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MABT886 Anti-VE-Cadherin Antibody, clone Vli37

This Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.

Anti-VE-Cadherin Antibody, clone Vli37 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Cdh5 UniProt Number: P55284
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MABT886
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, BWB, IH(P), ICCRbCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABT886
DescriptionAnti-VE-Cadherin Antibody, clone Vli37
Alternate Names
  • Cadherin-5
  • CD144
  • Vascular endothelial cadherin
  • VE-Cad
  • VE-cadherin
  • VEcad
Background InformationCadherin-5 (UniProt P55284; also known as Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad, CD144) is encoded by the Cdh5 gene (Gene ID 12562) in murine species. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin, which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal IgG in supernatant with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse brain endothelial bEnd.3 cell lysate.
Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected VE-cadherin expression in lysastes from mouse lung tissue, mouse brain endothelial bEnd.3 cells, and bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunohistochemistry Analysis: An 1:250-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in formalin-fixed, paraffin-embeded mouse lung, aorta, and liver tissue sections (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunocytochemistry Analysis: An 1:500-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Biological Information
ImmunogenLinear peptide corresponding to the C-terminal cytoplasmic domain sequence of mouse VE-Cadherin.
EpitopeNear C-terminus.
CloneVli37
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityClone Vli37 stains endothelial adherens junctions by immunocytochemistry and immunohistochemistry.
IsotypeIgG
Species Reactivity
  • Mouse
  • Bovine
Species Reactivity NoteMouse, Bovine. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cdh5
Purification MethodUnpurified
UniProt Number
Molecular Weight~110 observed. Target band size appears larger than the calculated molecular weight of 83.05 kDa due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT886 04055977331141

Documentation

Anti-VE-Cadherin Antibody, clone Vli37 SDS

Title

Safety Data Sheet (SDS) 

Anti-VE-Cadherin Antibody, clone Vli37 Certificates of Analysis

TitleLot Number
Anti-VE-Cadherin, clone Vli37 - 3419588 3419588
Anti-VE-Cadherin, clone Vli37 - 3694731 3694731
Anti-VE-Cadherin, clone Vli37 - 3896376 3896376
Anti-VE-Cadherin, clone Vli37 - 3902097 3902097
Anti-VE-Cadherin, clone Vli37 - 4048724 4048724
Anti-VE-Cadherin, clone Vli37 - 4048724A 4048724A
Anti-VE-Cadherin, clone Vli37 -Q2653601 Q2653601
Anti-VE-Cadherin, clone Vli37 Monoclonal Antibody 2973056
Anti-VE-Cadherin, clone Vli37 Monoclonal Antibody 3100457