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AB2251-I Anti-VGluT2 Antibody

Anti-VGluT2, Cat. No. AB2251-I is a Guinea pig polyclonal antibody that detects VGluT2 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.

Anti-VGluT2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: Slc17a6, Dnpi, Vglut2 UniProt Number: Q9JI12
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AB2251-I
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IHCGpSerumPolyclonal Antibody
Description
Catalogue NumberAB2251-I
DescriptionAnti-VGluT2 Antibody
Alternate Names
  • Vesicular glutamate transporter 2
  • Differentiation-associated BNPI
  • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte
  • Solute carrier family 17 member 6
  • VGluT2
Background InformationVesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID: 84487) in rat. VGluT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. It contains seven cytoplasmic domains, twelve transmembrane domains, and six vesicular domains. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. Expression of VGluT2 is reported to increase sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGluT1 and VGluT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGluT2 expression predominates. VGluT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1.
References
Product Information
FormatSerum
HS Code3002 15 90
PresentationGuinea Pig polyclonal antibody in buffer containing serum with 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-VGluT2, Cat. No. AB2251-I is a Guinea pig polyclonal antibody that detects VGluT2 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected VGluT2 in mouse brain and rat brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of rat Vesicular Glutamate Transporter 2 (VGluT2).
EpitopeC-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostGuinea Pig
SpecificityThis Guinea Pig polyclonal antibody detects Vesicular glutamate transporter 2. It targets an epitope within 18 amino acids from the C-terminal region.
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteRat, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slc17a6
  • Dnpi
  • Vglut2
Purification MethodUnpurified.
UniProt Number
Molecular Weight~56 kDa observed; 64.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat Brain Membrane tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in Rat Brain Membrane tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB2251-I 04055977355345