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MAB4308 Anti-VIN-IS-56 Antibody, clone VIN-IS-56

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Neural Stem Cells Gene Symbol: VIN-IS-56
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MAB4308
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MaFC, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4308
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-VIN-IS-56 Antibody, clone VIN-IS-56
Background InformationAlmost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.
References
Product Information
FormatPurified
Control
  • Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
PresentationPurified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide
Quality LevelMQ100
Applications
ApplicationAnti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunohistochemistry & Immunocytochemistry:

Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).

Recommended antibody dilution: 10-20 μg/ml

Dilution Buffer: PBS + 4% FCS

Expected staining pattern: cell surface staining



FACS Analysis:

Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells

Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde

Recommended controls: Undifferentiated human ES or EC cells stain weakly.



Immunoblotting:

Not tested



EIA:

Not tested



Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenHuman Embryonal carcinoma cells
CloneVIN-IS-56
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.
IsotypeIgM
Species Reactivity
  • Mammals
Antibody TypeMonoclonal Antibody
Gene Symbol
  • VIN-IS-56
Stem Cell Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB4308 04053252576263

Documentation

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 SDS

Title

Safety Data Sheet (SDS) 

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 Certificates of Analysis

TitleLot Number
MOUSE VIN-IS-56 [GD3] PURIFIED MONOCLONAL ANTIBODY - 2443397 2443397
MOUSE VIN-IS-56 [GD3] - 3709491 3709491
MOUSE VIN-IS-56 [GD3] -2575779 2575779
MOUSE VIN-IS-56 [GD3] -2890766 2890766

References

Reference overviewPub Med ID
Surface antigens of human embryonic stem cells: changes upon differentiation in culture
Draper J.S. et al.
J. Anat., 200 (Pt 3):249-258 (2002)  2002

12033729 12033729
Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours
Andrews P.W. et al.
Int. J. Cancer, 66(6):806-816 (1996)  1996

8647654 8647654
Differentiation antigens of human germ cell tumours: distribution of carbohydrate epitopes on glycolipids and glycoproteins analyzed using PDMP, an inhibitor of glycolipid synthesis
Fenderson B.A. et al.
European Urology, 23:30-37 (1993)  1993

7682952 7682952
Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR)
Andrews P.W. et al.
Differentiation, 43:131-138 (1990)  1990

2373286 2373286