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MABN2547-100UL Anti-VMAT1 Antibody, clone N440 21

Anti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.

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MABN2547-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABN2547-100UL
DescriptionAnti-VMAT1 Antibody, clone N440 21
Alternate Names
  • Chromaffin granule amine transporter
  • Solute carrier family 18 member 1
  • Vesicular amine transporter 1
  • VAT1
Background InformationChromaffin granule amine transporter (UniProt: Q8R090; also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, VMAT1, VAT1) is encoded by the Scl19a1 (also known as Vmat1) gene (Gene ID: 110877) in murine species. Vesicular monoamine transporters (VMATs) are involved in presynaptic storage and release of neurotransmitters. They are located in the presynaptic region and are involved in the transport of monoamines into storage vesicle, which are released upon arrival of an action potential. Two structurally related, but pharmacologically distinct, VMATs have been described (VMAT1 and VMAT2) that are encoded by separate genes. VMAT1 is a multi-pass membrane acidic glycoprotein that is found in both large dense-core vesicles and in small synaptic vesicles. It s both C- and N-terminal regions are located in the cytosolic side of the vesicle. VMAT1 is detected in adrenal medulla, and brain (at protein level). In brain, specifically found in the medulla oblongata, pons, prefrontal cortex, striatum, dentate gyrus and hippocampus. VMAT1 is shown to play a key role in survival of hippocampal neurons and may contribute to neurocognitive deficits observed in neuropsychiatric disorders. (Ref.: Multani, PK., et al. (2013). Neurosci. 232; 32-44).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Applications
ApplicationAnti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenRecombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
Epitopeextracellular domain
CloneN440/21
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Scl18a1
  • Vmat1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~93 kDa observed; 56.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat cerebellum tissue lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected VMAT1 in rat cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN2547-100UL 04054839636523

Documentation