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MABN812 Anti-VPS4A Antibody, clone 14B1.1

Detect VPS4A using this Anti-VPS4A Antibody, clone 14B1.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-VPS4A Antibody, clone 14B1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: VPS4A, VPS4 UniProt Number: Q9UN37
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MABN812
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN812
DescriptionAnti-VPS4A Antibody, clone 14B1.1
Alternate Names
  • Vacuolar protein sorting-associated protein 4A
  • Protein SKD2
  • VPS4-1
  • hVPS4
  • VPS4A
Background InformationVacuolar protein sorting-associated protein 4A (EC 3.6.4.6; UniProt Q9UN37; also known as hVPS4, Protein SKD2, SKD1-homolog, Vacuolar protein sorting factor 4A, Vacuolar sorting protein 4, VPS4A) is encoded by the VPS4A gene (also known as SKD1A, VPS4, VPS4-1) (Gene ID 27183) in human. Vps4 is a member of the ATPases associated with various cellular activities. The ESCRT (endosomal sorting complexes required for transport) machinery consists of five complexes (SCRT-0, ESCRT-I, ESCRT-II, ESCRT-III, and Vps4) that mediate multiple cellular membrane remodeling events, ranging from multivesicular bodies and exosomes formation to the final membrane abscission stage of cytokinesis, all of which converge in the scission of the narrow membrane neck that connects bud to parent membrane or daughter cell to daughter cell. Scission depends on the polymerization of soluble ESCRT-III monomers or dimers into tightly membrane-bound filaments. Vps4 is essential for ESCRT function in cells and it mediates the disassembly of ESCRT-III filaments to ensure a constant replenishing of the pool of soluble ESCRT-III monomers. The ESCRTs are also exploited by HIV-1 and many other enveloped viruses for their escape from the host cells. Vps4A is reported to facilitate the secretion of oncogenic miRNAs in exosomes and downregulation of Vps4A in hepatocellular carcinoma (HCC) is associated with tumor progression and metastasis.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect VPS4A using this Anti-VPS4A Antibody, clone 14B1.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected VPS4A in 200 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VPS4A in rat cerebral cortex tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human VPS4A sequence at the N-terminus.
EpitopeNear N-terminus
Clone14B1.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • VPS4A
  • VPS4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~50 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VPS4A in 200 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN812 04055977173499

Documentation

Anti-VPS4A Antibody, clone 14B1.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-VPS4A Antibody, clone 14B1.1 Certificates of Analysis

TitleLot Number
Anti-VPS4A, clone 14B1.1 - 3754089 3754089
Anti-VPS4A, clone 14B1.1 - 3899547 3899547
Anti-VPS4A, clone 14B1.1 - 4001596 4001596
Anti-VPS4A, clone 14B1.1 -Q2574771 Q2574771

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.