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CP74 Anti-Vinculin Mouse mAb (V284 or VLN01)

This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP74
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
CP74-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
Catalogue NumberCP74
Brand Family Calbiochem®
Application Data
Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesPalovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
Bakolitsa, C., et al. 1999 Cell 99, 603.
Rudiger, M., et al. 1998 Bioessays 20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Negative controlNormal mouse serum
Positive controlSkin cells or tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
Immunoprecipitation (2 µg/ml lysate)
Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
Application CommentsThis antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogensemi-purified, human vinculin
ImmunogenHuman
CloneV284 or VLN01
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CP74-100UG 04055977221022

Documentation

Anti-Vinculin Mouse mAb (V284 or VLN01) SDS

Title

Safety Data Sheet (SDS) 

Anti-Vinculin Mouse mAb (V284 or VLN01) Certificates of Analysis

TitleLot Number
CP74

References

Reference overview
Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
Bakolitsa, C., et al. 1999 Cell 99, 603.
Rudiger, M., et al. 1998 Bioessays 20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
Immunoprecipitation (2 µg/ml lysate)
Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
Application Data
Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
DescriptionProtein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
BackgroundVinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and α-actinin, which in turn binds to actin. Also, vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor. Vinculin has been associated with tumor suppressor activity. Vinculin is widely distributed in tissues and expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix. Vinculin has been found in all adherens junctions, while the larger splice variant, meta-vinculin, is found only in smooth and cardiac muscle.
HostMouse
Immunogen speciesHuman
Immunogensemi-purified, human vinculin
CloneV284 or VLN01
IsotypeIgG₁
Specieshuman
Positive controlSkin cells or tissue
Negative controlNormal mouse serum
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesPalovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
Bakolitsa, C., et al. 1999 Cell 99, 603.
Rudiger, M., et al. 1998 Bioessays 20, 733
Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.