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MABF2234-25UG Anti-West Nile Virus Antibody, clone E16

MABF2234-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2234-25UG
DescriptionAnti-West Nile Virus Antibody, clone E16
Alternate Names
  • E protein
  • Envelope protein
  • Env
Background InformationWest Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. The envelope glycoprotein (E) undergoes a conformational rearrangement triggered by low pH that results in a class II fusion event required for viral entry. The E protein contains a three-domain structure where domain I is an eight-stranded -barrel in the center portion of the protein (aa 1-51, 134-195, and 284-297). The DII domain consists of 170 residues inserted into DI (aa 52-133 and 196-283). At the distal end of DII domain is a fusion loop (aa 98-110) and a conserved glycine-rich, hydrophobic sequence, which is important for flaviviral fusion. The third domain, DIII, is an Ig-like domain with seven -strand and has been implicated in receptor binding. The E protein also has a single N-linked glycosylation site at Asn 154, located within the ectodomain of prM domain and is cleaved by cellular enzymes during viral particle maturation. Clone E16 specifically binds to the DIII lateral ridge and displays high neutralizing activity and is reported to completely inhibit infection even at 10 ng/mL. (Ref.: Nybakken, GE et al. (2006). J. Virol. 80(23); 11467-11474; Oliphant, T., et al. (2006). J. Virol. 80(24); 12149-12159).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
Applications
ApplicationAnti-West Nile Virus, clone E16, Cat. No. MABF2234, is a highly specific mouse monoclonal antibody that targets WNV envelope protein and has been tested for use in Immunocytochemistry, Neutralizing Applications, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Neutralizing
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).

Neutralizing Analysis: A representative lot completely inhibited West Nile virus infection at 10 ng/mL. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Biological Information
ImmunogenWest Nile Virus envelope protein.
CloneE16
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone E16 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DIII lateral ridge region.
IsotypeIgG2bκ
Species Reactivity
  • Virus
Species Reactivity NoteVirus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • E
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with Full-length ectodomain of West Nile Virus envelope protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected Full-length ectodomain of West Nile Virus envelope protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2234-25UG 04061841039112

Documentation

Anti-West Nile Virus Antibody, clone E16 SDS

Title

Safety Data Sheet (SDS) 

Anti-West Nile Virus Antibody, clone E16 Certificates of Analysis

TitleLot Number
Anti-West Nile Virus, clone E16 - 3277845 3277845

References

Reference overviewPub Med ID
A therapeutic antibody against west nile virus neutralizes infection by blocking fusion within endosomes.
Thompson, BS; Moesker, B; Smit, JM; Wilschut, J; Diamond, MS; Fremont, DH
PLoS Pathog  5  e1000453  2009

Show Abstract
19478866 19478866
Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein.
Oliphant, T; Nybakken, GE; Engle, M; Xu, Q; Nelson, CA; Sukupolvi-Petty, S; Marri, A; Lachmi, BE; Olshevsky, U; Fremont, DH; Pierson, TC; Diamond, MS
J Virol  80  12149-59  2006

Show Abstract
17035317 17035317