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MAB8152-I-100UL Anti-West Nile Virus/Kunjin Antibody, NS1 Antibody, clone 3.1112G

Anti-West Nile Virus/Kunjin, NS1, clone 3.1112G, Cat. No. MAB8152-I, is a mouse monoclonal antibody that detects NS1 protein of West Nile virus and is tested for use in Affinity Binding Assay, ELISA, Immunofluorescence, and Western Blotting.

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MAB8152-I-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMAB8152-I-100UL
DescriptionAnti-West Nile Virus/Kunjin Antibody, NS1 Antibody, clone 3.1112G
Alternate Names
  • West Nile Virus NS1 Protein
  • WNV NS1 Protein
  • Genome Polyprotein
Background InformationGenome polyprotein (UniProt: P14335) is encoded by the POLY gene (Gene ID: 912267) in West Nile virus. West Nile virus (WNV) is a mosquito-borne positive-sense RNA virus of the Flavivirus genus within the Flaviviridae family. WNV genome encodes for three structural domains known as C, prM, and E and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5). These are translated as a single polypeptide, which is subsequently cleaved by viral and cellular proteases. NS1 (aa 792-1143) is a glycoprotein that is involved in immune evasion, pathogenesis, and viral replication. It is secreted from infected cells and may vary its oligomeric state between monomers, dimers (the primary form) and hexamers. Once cleaved off the polyprotein, it is targeted to three destinations: the viral replication cycle, the plasma membrane, and the extracellular compartment. Intracellular NS1 functions as an essential cofactor for viral replication, and it localizes to WNV replication sites, whilst cell surface and secreted NS1 act as immunomodulators. NS1 is required for formation of the replication complex and recruitment of other non-structural proteins to the ER-derived membrane structures. It is reported to antagonize the complement function and inhibit signal transduction originating from Toll-like receptor 3. (Ref.: Martín-Acebes MA, et al. (2012). World J Virol. 1(2); 51-70).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-West Nile Virus/Kunjin, NS1, clone 3.1112G, Cat. No. MAB8152-I, is a mouse monoclonal antibody that detects NS1 protein of West Nile virus and is tested for use in Affinity Binding Assay, ELISA, Immunofluorescence, and Western Blotting.
Key Applications
  • ELISA
  • Affinity Binding Assay
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

ELISA Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in ELISA applications (Shelite, T.R., et al. (2008). Vector Borne Zoonotic Dis.;8(3):321-9; Ledermann, J.P., et al. (2011). Clin Vaccine Immunol.;18(4):580-7; Thompson, N.N., et al. (2012). Vector Borne Zoonotic Dis.;12(11):969-78).

Affinity Binding Assay: A representative lot of this antibody bound West Nile Virus/Kunjin with a KD of 1.0 x 10-12 in an affinity binding assay.

Western Blotting Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in Western Blotting applications (Whiteman, M.C., et al. (2011). Vaccine.;29(52):9702-10).

Immunofluorescence Analysis: A representative lot detected West Nile Virus/Kunjin, NS1 in Immunofluorescence applications (Garcia-Tapa, D. et al. (2006). Vet Immunol Immunopathol.;110(3-4):229-44; Giel-Moloney, M., et al. (2019). Sci Rep.;9(1):20005).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenWest Nile Virus (WNV; subtype KUNV) (isolate OR393).
EpitopeExtracellular domain
Clone3.1112G
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3.1112G is a mouse monoclonal antibody that detects West Nile virus NS1 protein.
IsotypeIgM
Species Reactivity
  • Virus
Species Reactivity NoteWest Nile virus.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLY
Purification MethodLigatrap
UniProt Number
Molecular Weight381.37 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant West Nile Virus NS1 protein.

ELISA Analysis: Various concentrations of this antibody detected recombinant West Nile virus NS1 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB8152-I-100UL 04065266791495