Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE542 Anti-YTHDF2 Antibody

This Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2.

Anti-YTHDF2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: YTHDF2, HGRG8 UniProt Number: Q9Y5A9
View Products on Sigmaaldrich.com
ABE542
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE542
DescriptionAnti-YTHDF2 Antibody
Alternate Names
  • YTH domain-containing family protein 2
  • CLL-associated antigen KW-14
  • High-glucose-regulated protein 8
  • Renal carcinoma antigen NY-REN-2
Background InformationYTH domain-containing family protein 2 (YTHDF2), also known as CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, is also encoded by the gene name YTHDF2 and HGRG8. YTHDF2 recognizes specifically and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs. Recent studies have shown that YTHDF2 may play an important role in the efficiency of mRNA splicing, processing and stability. Additionally, YTHDF2 has been shown to act as a regulator of mRNA stability. And in the process of binding to m6A-containing mRNAs, YTHDF2 has been implicated to localize to mRNA decay sites, such as processing bodies (P-bodies), which leads to mRNA degradation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 15 µg/mL from a representative lot detected YTHDF2 in WT HeLa cell lysate and YTHDF2 overexpressing lysate (Chuan He, Univerity of Chicago).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the localization of mRNA processing bodies (P-bodies) to human YTHDF2.
EpitopeLocalization to mRNA processing bodies (P-bodies)
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • YTHDF2
  • HGRG8
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~62 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected YTHDF2 in 10 µg of C6 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE542 04055977122367