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MABT888 Anti-ZO-2 Antibody, clone Vli13

Detect ZO-2 using this Anti-ZO-2 Antibody, clone Vli13 validated for use in Immunohistochemistry (Paraffin).

Anti-ZO-2 Antibody, clone Vli13 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Tjp2, Zo2 UniProt Number: Q9Z0U1
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MABT888
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIH(P)RbCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABT888
DescriptionAnti-ZO-2 Antibody, clone Vli13
Alternate Names
  • Tight junction protein ZO-2
  • Tight junction protein 2
  • Zona occludens protein 2
  • Zonula occludens protein 2
  • ZO-2
Background InformationTight junction protein ZO-2 (UniProt Q9Z0U1; also known as Tight junction protein 2, Zona occludens protein 2, Zonula occludens protein 2) is encoded by the Tjp2 (also known as Zo2) gene (Gene ID 21873) in murine species. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, -2, and -3 found at the submembranous domain of TJs in epithelia and endothelia. The 160 kDa ZO-2 contains three PDZ domains (PDZ1/a.a. 10-97, PDZ2/a.a. 287-365, PDZ3/a.a. 489-570) at its N-terminal end, followed by an SH3 domain (a.a. 584-649), a GUK/GK homology domain (a.a. 660-858), and a short PDZ-binding motif (TEL; a.a. 1165-1167) at the C-terminus. In addition to associating with various transmembrane junction proteins via its C-terminal PDZ-binding motif, ZO-2 is also involved in modulating gene expression and cell proliferation by interacting with a number of proteins in the nucleus. Evidence suggests that ZO-2 is also involved in stress response and cytoprotective mechanisms.
References
Product Information
FormatCulture Supernatant
PresentationRabbit monoclonal antibody in tissue culture supernatant with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect ZO-2 using this Anti-ZO-2 Antibody, clone Vli13 validated for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ZO-2 in human heart and spleen tissue sections.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected ZO-2 in rat heart myocardium and brain tissue sections (Courtesy of Dr. Volkhard Lindner, Tufts University School of Medicine, ME, USA).
Biological Information
ImmunogenLinear peptide corresponding to a C-terminal sequence of mouse ZO-2.
EpitopeC-terminal region.
CloneVli13
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with human ZO-2 spliced isoforms A1, A2, C1, C2, 6, and 7, but not A3, reported by UniProt (Q9UDY2).
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tjp2
  • Zo2
Purification MethodUnpurified
UniProt Number
Molecular Weight134.0 kDa (isoform A1), 117.8 kDa (isoform A2), 131.4 kDa (isoform C1), 115.2 kDa (isoform C2), 130.4 kDa (isoform 6), and 137.3 kDa (isoform 7) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ZO-2 in mouse heart tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT888 04055977315769