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MABE1142 Anti-ZP-2 Antibody, clone IE-3

Anti-ZP-2, clone IE-3, Cat. No. MABE1142, is a highly specific rat monoclonal antibody that targets Zona pellucida sperm-binding protein 2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

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MABE1142
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1142
DescriptionAnti-ZP-2 Antibody, clone IE-3
Alternate Names
  • Zona pellucida sperm-binding protein 2
  • Zona pellucida glycoprotein 2
  • Zona pellucida protein A
Background InformationZona pellucida sperm-binding protein 2 (UniProt: P20239; also known as Zona pellucida glycoprotein 2, Zp-2, Zona pellucida protein A) is encoded by the Zp2 (also known as Zp-2, Zpa) gene (Gene ID: 22787) in murine species. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The mammalian zona pellucida mediates species-specific sperm binding, induction of the acrosome reaction, and prevents post-fertilization polyspermy. ZP2 is expressed during the 2-week growth phase of oogenesis, prior to ovulation and may act as a secondary sperm receptor and is proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL and is exocytosed from cortical granules after fertilization, yielding an N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage plays an important role in the post-fertilization block to polyspermy. Female mice lacking Zp2 gene do not form a stable zona matrix and are sterile. (Ref.: Hinsch, KD and Hinsch, E. (1999). Andrologia.31(5):320-322.)
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ZP-2, clone IE-3, Cat. No. MABE1142, is a highly specific rat monoclonal antibody that targets Zona pellucida sperm-binding protein 2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Immunofluorescence
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot detected ZP-2 in Immunoprecipitation applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).

Immunofluorescence Analysis: A representative lot detected ZP-2 in Immunofluorescence applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800).

Immunofluorescence A representative lot detected ZP-2 in Immunofluorescence applications (Avella, M.A., et. al. (2016). Sci Transl Med. 8(336):336ra60).

ELISA Analysis: A representative lot detected ZP-2 in Western Blotting applications (East, I.J., et. al. (1984). J Cell Biol. 98(3):795-800; Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).

Western Blotting Analysis: A representative lot detected ZP-2 in ELISA applications (Sun, W., et. al. (1999). Biol Reprod. 60(4):900-7).
Biological Information
ImmunogenPurified mouse zonae pellucidae.
EpitopeN-terminus
CloneIE-3
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone IE-3 detects Zona pellucida sperm-binding protein 2 in murine species.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Zp2
  • Zp-2
  • Zpa
Purification MethodProtein G purified
UniProt Number
Molecular Weight~120 kDa observed; 80.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse ovary tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ZP-2 in 10 µg of mouse ovary tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1142 04054839233128

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