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ABE1958 Anti-Zic1/2

Anti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.

Anti-Zic1/2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Zic 1, Zic UniProt Number: P46684
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ABE1958
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIF, WB, ChIP, ICCRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE1958
DescriptionAnti-Zic1/2
Alternate Names
  • Zinc finger protein of the cerebellum 1
Background InformationZinc finger protein ZIC 1 (UniProt: P46684; also known as Zinc finger protein of the cerebellum 1) is encoded by the Zic 1 (also known as Zic) gene (Gene ID: 22771) in murine species. Zic 1 is a member of the GLI C2H2-type zinc-finger protein family. Zic 1 is expressed in the central nervous system and in osteoblasts. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. It acts as a transcriptional activator that is involved in neurogenesis and plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. The C2H2-type 3, 4 and 5 zinc finger domains of Zic 1 are considered to be essential for transcription activation and for Zic 1 interaction with MyoD family inhibitor domain-containing (MDFIC) protein. Zic 1 is also shown to be involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). It plays a role in the regulation of MF axon pathway choice and promotes MF migration towards ipsilaterally-located cerebellar territories.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
Application NotesChromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Zic1/2 in mouse cerebellum (Frank, C.L., et. al. (2015). Nat Neurosci. 18(5):647-56).

Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. al. (2009). J Neurosci. 29(23):7439-49).

Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. al. (2000). Mol Cell Biol. 20(23):9055-67).
Biological Information
ImmunogenA linear peptide corresponding to 20 amino acids from the C-terminal end of mouse Zic 1 protein.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Zic1 protein in mouse and human.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Zic 1
  • Zic
Purification MethodUnpurified
UniProt Number
Molecular Weight~53 kDa observed; 48.33 kDa calculated for Zic1; 54.95 kDa calculated for Zic2. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1958 04054839168697