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07-2184 Anti-acetyl-GAPDH (Lys160) Antibody

This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).

Anti-acetyl-GAPDH (Lys160) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: GAPDH, GAPD UniProt Number: P04406
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07-2184
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIA, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-2184
DescriptionAnti-acetyl-GAPDH (Lys160) Antibody
Alternate Names
  • Glyceraldehyde-3-phosphate dehydrogenase
  • GAPDH
  • Peptidyl-cysteine S-nitrosylase GAPDH
Background InformationAcetyl-GAPDH (Lys160), also known as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH(lys160), or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene GAPDH/GAPD, is an interesting multifunctional metabolic enzyme that plays a critical role in glycolysis but also many other important nuclear and cell functions such as transcription, RNA transport, DNA replication and even apoptosis and cell death. In glycolysis, GAPDH is a key enzyme that catalyzes the first step in the pathway by converting G3P into 3-phospho-D glyceroyl phosphate. An example of GAPDH’s regulation of gene transcription as a component of a GAIT complex, which selectively inhibits the activation of various gamma interferon induced inflammation responses. The protein-protein interactions of GAPDH are at least partially controlled by acetylation. For instance, transport of GAPDH into the nucleus is dependent upon acetylation at lysines 117, 227, and 251, and acetylation at lysine 183 governs co-factor interactions. Acetylation of GAPDH at lysine 160 ultimately is involved in p53 mediated cell death.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
  • Immunoprecipitation
Application NotesPeptide Inhibition Analysis: 1 µg/mL from a representative lot detected acetyl GAPDH (Lys60) in LPS/IFNgamma in RAW264.7 nuclear extract.

Immunoprecipitation Analyis: A representative lot immunoprecipitated acetyl GAPDH (Lys60) from LPS/IFNgamma treated RAW264.7 cell lysate (Prof. Akira Sawa, Johns Hopkins University, Baltimore.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the human GAPDH acetylated at Lys160.
Epitope0
Concentration0.5 mg/mL
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Non-Human Primate, Bovine, Horse, Feline, Sheep, Porcine, and Canine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GAPDH
  • GAPD
Modifications
  • Acetylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~37 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in untreated and LPS/IFNgamma treated RAW264.7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl GAPDH (Lys60) in LPS/IFNgamma treated RAW264.7 cell lysate and not in untreated RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-2184 04053252971167