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06-1372 Sigma-AldrichAnti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Technical Information

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H1E, H1F4 UniProt Number: P10412

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, DB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	06-1372
Description	Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody
Alternate Names	Histone H1.4 Histone H1b
Background Information	H1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.

References

Product Information
Format	Affinity Purified
Control	Colcemid treated HeLa acid extract
Presentation	Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.
Key Applications	Western Blotting Dot Blot
Application Notes	Dot Blot Analysis: A 1:1,320 dilution from representative lot detected acetyl (Lys26) phospho (Ser27) Histone H1.4 peptides.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
Epitope	N-terminus
Host	Rabbit
Specificity	This antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with Human. Predicted to react with Canine, Orangutan, Giant Panda, and Rhesus Macaque based on 100% sequence homology. Other homologies: Rat and Mouse (82% sequence homology).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_005312
Gene Symbol	HIST1H1E H1F4
Purification Method	Affinity Purfied
UniProt Number	P10412
Molecular Weight	~35 kDa observed. Uncharacterized bands may be observed at ~70 kDa and ~17 kDa in some acid extracts.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in colcemid treated HeLa acid extract. Western Blot Analysis: A 1:3,000 dilution of this antibody detected acetyl (Lys26) phospho (Ser27) Histone H1.4 in 10 µg of colcemid treated HeLa acid extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
06-1372	04053252523212

Documentation

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody Certificates of Analysis

Title	Lot Number
Anti-acetyl (Lys26) phospho (Ser27) -2780929	2780929
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 2451326	2451326
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 2467145	2467145
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - Q2032349	Q2032349

References

Reference overview	Pub Med ID
Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. Terme, Jean-Michel, et al. FEBS Lett., (2014) 2014 Show Abstract In mammals, the linker histone H1, involved in DNA packaging into chromatin, is represented by a family of variants. H1 tails undergo post-translational modifications (PTMs) that can be detected by mass spectrometry. We developed antibodies to analyze several of these as yet unexplored PTMs including the combination of H1.4 K26 acetylation or trimethylation and S27 phosphorylation. H1.2-T165 phosphorylation was detected at S and G2/M phases of the cell cycle and was dispensable for chromatin binding and cell proliferation; while the H1.4-K26 residue was essential for proper cell cycle progression. We conclude that histone H1 PTMs are dynamic over the cell cycle and that the recognition of modified lysines may be affected by phosphorylation of adjacent residues.	24873882

Reference overview

Pub Med ID

Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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