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MABT522 Anti-alpha-Tubulin Antibody, clone RM113

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Anti-alpha-Tubulin Antibody, clone RM113 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: TUBA1B UniProt Number: P68363
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MABT522
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT522
DescriptionAnti-alpha-Tubulin Antibody, clone RM113
Alternate Names
  • Tubulin alpha-1B chain
  • Alpha-tubulin ubiquitous
  • Tubulin K-alpha-1
  • Tubulin alpha-ubiquitous chain
  • alpha-Tubulin
Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
References
Product Information
FormatPurified
PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.
Biological Information
ImmunogenA peptide corresponding to the C-terminus of α-Tubulin.
EpitopeC-terminus
CloneRM113
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • TUBA1B
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT522 04055977181548

Documentation

Anti-alpha-Tubulin Antibody, clone RM113 Certificates of Analysis

TitleLot Number
Anti-alpha-Tubulin, clone RM113 - VP1903091 VP1903091
Anti-alpha-Tubulin, clone RM113 - VP2202183 VP2202183
Anti-alpha-Tubulin, clone RM113 - VP2310033 VP2310033
Anti-alpha-Tubulin, clone RM113 - VP2409055 VP2409055
Anti-alpha-Tubulin, clone RM113 -QVP1503208 QVP1503208
Anti-alpha-Tubulin, clone RM113 Monoclonal Antibody VP1706196

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.