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MABN254 Sigma-AldrichAnti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3

Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3 is an antibody against amyloid beta peptide (MOAB-2), pan, clone 6C3 for use in western blotting, IHC (Paraffin), Immunofluorescence, IP, Dot Blot.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: APP, A4, AD1 UniProt Number: P05067

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IH(P), IF, IP, DB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN254
Description	Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3
Alternate Names	Amyloid beta A4 protein ABPP APPI APP Alzheimer's disease amyloid protein cerebral vascular amyloid peptide CVAP PreA4 Protease nexin-II PN-II
Background Information	Pan-amyloid-beta (1-40) is one of several functional peptides (39 to 43 amino acids in length) formed from proteolytic cleavage of the amyloid-beta precursor protein (APP) by the family of secretase enzymes. Although the APP protein is required for a number of biological functions, including axonal transport, cell adhesion, and transcription, the Ab 1-40 peptide has been studied mostly for its potential to aggregate and form neurite plaques, which may contribute to neurotoxicity, particularly in the context of neurodegenerative disease such as Alzheimer’s. Previous studies have demonstrated that Anti-pan amyloid beta peptide (MOAB2), clone 6C3 detects specifically amyloid-beta, but not APP. Furthermore MOAB2 detects multiple amyloid-beta 40 and amyloid-beta 42 conformations including unaggregated, oligomeric and fibrillar. In a number of immunohistochemical and biochemical analyses, MOAB2 consistently detected intraneuronal amyloid-beta, but not APP, and showed greater specificity to amyloid-beta than 6E10. MOAB2 is therefore suitable for sensitive and specific detection of accumulating amyloid-beta peptides in Alzheimer’s disease models.

References

Product Information
Format	Purified
Control	Alzheimer's diseased human brain tissue.
Presentation	Purified mouse monoclonal IgG2aλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3 is an antibody against amyloid beta peptide (MOAB-2), pan, clone 6C3 for use in western blotting, IHC (Paraffin), Immunofluorescence, IP, Dot Blot.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Immunofluorescence Immunoprecipitation Dot Blot
Application Notes	Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta Peptide in human Alzeimer's diseased brain (see website for images). Western Blot Analysis: A representative lot was used by an independent laboratory in unaggregated forms of Aß42 and Aß40. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.) Dot Blot Analysis: Serial Aβ40 and Aβ42 dilutions were probed with MOAB-2 or 6E10 by an independent laboratory. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.) Immunoprecipitationt Analysis: A representative lot was used by an independent laboratory in unaggregated and fibrillar forms of Aß42 and Aß40. (Youmans, K.L., et al. (2012). Mol Neurodegener. 7;8.)

Biological Information
Immunogen	Linear peptide corresponding to human pan amyloid beta peptide (MOAB-2).
Clone	6C3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes amyloid-ß and not APP (amyloid precursor protein). Specifically, clone 6C3 recognizes unaggregated, oligomeric, and fibrillar forms of Aß42 and Aß40.
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000475
Gene Symbol	APP A4 AD1
Purification Method	Protein G Purified
UniProt Number	P05067
Molecular Weight	4 kDa calculated but could be larger since clone 6C3 recognizes unaggregated, oligomeric, and fibrillar forms of Aß42 and Aß40.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in Alzheimer's diseased human brain tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Amyloid Beta Peptide in human Alzheimer's diseased brain tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MABN254	04053252698507

Documentation

Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3 SDS

Title
Safety Data Sheet (SDS)

Anti-amyloid beta peptide (MOAB-2), pan Antibody, clone 6C3 Certificates of Analysis

Title	Lot Number
Anti-amyloid beta peptide (MOAB-2), -2613128	2613128
Anti-amyloid beta peptide (MOAB-2), -2660168	2660168
Anti-amyloid beta peptide (MOAB-2), -2793610	2793610
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 2430547	2430547
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 2219403	2219403
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 2298456	2298456
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 3246198	3246198
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 3434650	3434650
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 3609982	3609982
Anti-amyloid beta peptide (MOAB-2), pan, clone 6C3 - 3713390	3713390

References

Reference overview	Pub Med ID
Intraneuronal Aβ detection in 5xFAD mice by a new Aβ-specific antibody. Youmans, Katherine L, et al. Mol Neurodegener, 7: 8 (2012) 2012 Show Abstract The form(s) of amyloid-β peptide (Aβ) associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal Aβ accumulation is an issue of considerable controversy; even the existence of Aβ deposits within neurons has recently been challenged by Winton and co-workers. These authors purport that it is actually intraneuronal APP that is being detected by antibodies thought to be specific for Aβ. To further address this issue, an anti-Aβ antibody was developed (MOAB-2) that specifically detects Aβ, but not APP. This antibody allows for the further evaluation of the early accumulation of intraneuronal Aβ in transgenic mice with increased levels of human Aβ in 5xFAD and 3xTg mice.	22423893

Reference overview

Pub Med ID

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