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MABT523 Anti-beta-Actin Antibody, clone RM112

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Anti-beta-Actin Antibody, clone RM112 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ACTB UniProt Number: P60709
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MABT523
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT523
DescriptionAnti-beta-Actin Antibody, clone RM112
Alternate Names
  • Actin
  • cytoplasmic 1
  • Beta-actin
  • beta-Actin
Background InformationActin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show > 90% amino-acid (aa) homology between isotypes and > 98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.
Biological Information
ImmunogenA peptide corresponding to the N-terminus of beta-Actin.
EpitopeN-terminus
CloneRM112
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ACTB
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT523 04055977181555

Documentation

Anti-beta-Actin Antibody, clone RM112 Certificates of Analysis

TitleLot Number
Anti-beta-Actin, clone RM112 -VP1606030 VP1606030
Anti-beta-Actin, clone RM112 - VP1907262 VP1907262
Anti-beta-Actin, clone RM112 - VP2001075 VP2001075
Anti-beta-Actin, clone RM112 - VP2008070 VP2008070
Anti-beta-Actin, clone RM112 - VP2106251 VP2106251
Anti-beta-Actin, clone RM112 - VP2109163 VP2109163
Anti-beta-Actin, clone RM112 - VP2111182 VP2111182
Anti-beta-Actin, clone RM112 - VP2201262 VP2201262
Anti-beta-Actin, clone RM112 - VP2209020 VP2209020
Anti-beta-Actin, clone RM112 - VP2310035 VP2310035
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.