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MABE1828-25UL Anti-beta-Catenin Antibody, clone mAb-Cat1

Anti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunoprecipitation, and Western Blottiing.

Anti-beta-Catenin Antibody, clone mAb-Cat1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CTNNB1, CTNNB, OK/sw-cl.35, PRO2286 UniProt Number: P35222
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MABE1828-25UL
25 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HChIP, DB, IP, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1828-25UL
DescriptionAnti-beta-Catenin Antibody, clone mAb-Cat1
Alternate Names
  • beta-Catenin 1
  • Catenin beta-1
Background InformationCatenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunoprecipitation, and Western Blottiing.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Dot Blot
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Biological Information
ImmunogenBSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
ClonemAb-Cat1
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityThis rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine and Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CTNNB1
  • CTNNB
  • OK/sw-cl.35
  • PRO2286
Purification MethodProtein G purified
UniProt Number
Molecular Weight~100 kDa observed; 85.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in SW 480 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected beta-Catenin in SW 480 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1828-25UL 04054839589683