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OP39 Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)

Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2

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OP39
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
OP39-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.
Catalogue NumberOP39
Brand Family Calbiochem®
SynonymsAnti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
References
ReferencesDiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
Bargmann, C.I., et al. 1968. Nature 319, 226.
Product Information
FormLiquid
FormulationIn 50 mM PBS, 0.2% gelatin, pH 7.5.
Negative controlHBL 100 cells
Positive controlSK-BR-3 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Immunoprecipitation, Immunofluorescence, Flow Cytometry Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. McKenzie, S.J., et al. 1989. Oncogene 4, 543. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. Not for Neutralization Studies McKenzie, S.J., et al. 1989. Oncogene 4, 543.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Neutralization Studies
Application NotesFlow Cytometry (5 µg/ml, see application references)
Frozen Sections (5 µg/ml)
Immunoblotting (see application references)
Immunofluorescence (5 µg/ml, see application references)
Immunoprecipitation (1 µg/sample, see application references)
Neutralization Studies (not recommended, see comments and application references)
Application CommentsRecognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogencells overexpressing human c-ErbB2/c-Neu
ImmunogenHuman
EpitopeWithin the extracellular domain
CloneTA-1
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
OP39-100UG 04055977227383

Documentation

Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1) Certificates of Analysis

TitleLot Number
OP39

References

Reference overview
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
Bargmann, C.I., et al. 1968. Nature 319, 226.

Citations

Title
  • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    SynonymsAnti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2
    ApplicationFlow Cytometry (5 µg/ml, see application references)
    Frozen Sections (5 µg/ml)
    Immunoblotting (see application references)
    Immunofluorescence (5 µg/ml, see application references)
    Immunoprecipitation (1 µg/sample, see application references)
    Neutralization Studies (not recommended, see comments and application references)
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.
    BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene.
    HostMouse
    Immunogen speciesHuman
    Immunogencells overexpressing human c-ErbB2/c-Neu
    EpitopeWithin the extracellular domain
    CloneTA-1
    IsotypeIgG₁
    Specieshuman, not mouse, not rat
    Positive controlSK-BR-3 cells
    Negative controlHBL 100 cells
    FormLiquid
    FormulationIn 50 mM PBS, 0.2% gelatin, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsRecognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesDiFiore, P.P., et al. 1987. Science 237, 178.
    Slamon, D.J., et al. 1987. Science 235, 177.
    Varley, J.M., et al. 1987. Oncogene 1, 423.
    Yamamoto, T., et al. 1986. Nature 319, 230.
    Blick, M., et al. 1984. Blood 64, 1234.
    Schwab, M., et al. 1984 Cold Spring Harbor Laboratory 2, 215.
    Bargmann, C.I., et al. 1968. Nature 319, 226.
    Citation
  • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
    		•
    Application referencesOriginal Clone, Immunoprecipitation, Immunofluorescence, Flow Cytometry Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. McKenzie, S.J., et al. 1989. Oncogene 4, 543. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650. Not for Neutralization Studies McKenzie, S.J., et al. 1989. Oncogene 4, 543.