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ABC457 Sigma-AldrichAnti-dUTPase Antibody

Anti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting.

Anti-dUTPase Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DUT UniProt Number: P33316

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Purified	Polyclonal Antibody

Description
Catalogue Number	ABC457
Description	Anti-dUTPase Antibody
Alternate Names	Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial dUTPase dUTP pyrophosphatase dUTPase
Background Information	dUTPase, also known as dUTP pyrophosphatase, and encoded by the gene DUT, is a critical enzyme involved in nucleotide metabolism for the production of dUMP, the immediate precursor of thymidine nucleotides. dUTPase normally can also function to decrease the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA and cause mutations. dUTPase is localized to the nucleus and mitochondrial locations. dUTPase is expressed in numerous tissues and has multiple isoforms. Some isoforms are constitutively expressed while others match DNA replication cycles. Because dUTPase plays a pivotal role in regulating cellular dUTP pools, this enzyme could have profound effects on the efficacy of agents that target thymidylate biosynthesis and dUTPase expression is being examined as a prognostic marker for overall survival and response to therapy in colon and other cancers where thymidylate therapy is used.

References

Product Information
Format	Purified
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-dUTPase Antibody is a highly specific rabbit polyclonal antibody, that targets dUTPase & has been tested in western blotting.
Key Applications	Western Blotting
Application Notes	Western Blotting Analysis: 2 µg/mL from a representative lot detected dUTPase in 10 µg of A431, HEK293, and HeLa cell lysates and human placenta tissue lysate. Western Blotting Analysis: A representative lot from an independent laboratory detected dUTPase in MDA-MB231, MCF-7, H184B5F5/M10 cell lysate (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).

Biological Information
Immunogen	Recombinant protein corresponding to human dUTPase.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_001020419
Gene Symbol	DUT
Purification Method	Protein A Purfied
UniProt Number	P33316
Molecular Weight	~18 kDa observed. The expected molecular weight of this protein is 27 kDa. However, this protein has been observed at 18 kDa (Prof. Z. F. Chang, National Yang-Ming University). Uncharacterized band(s) may be observed in some cell lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in MDA-MB-231 cell lysate. Western Blotting Analysis: 2 µg/mL of this antibody detected dUTPase in 10 µg of MDA-MB-231 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
ABC457	04053252939549

Documentation

Anti-dUTPase Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-dUTPase Antibody Certificates of Analysis

Title	Lot Number
Anti-dUTPase - 3504724	3504724
Anti-dUTPase - Q2341231	Q2341231

References

Reference overview	Pub Med ID
Tumor cells require thymidylate kinase to prevent dUTP incorporation during DNA repair. Hu, Chun-Mei, et al. Cancer Cell, 22: 36-50 (2012) 2012 Show Abstract The synthesis of dTDP is unique because there is a requirement for thymidylate kinase (TMPK). All other dNDPs including dUDP are directly produced by ribonucleotide reductase (RNR). We report the binding of TMPK and RNR at sites of DNA damage. In tumor cells, when TMPK function is blocked, dUTP is incorporated during DNA double-strand break (DSB) repair. Disrupting RNR recruitment to damage sites or reducing the expression of the R2 subunit of RNR prevents the impairment of DNA repair by TMPK intervention, indicating that RNR contributes to dUTP incorporation during DSB repair. We identified a cell-permeable nontoxic inhibitor of TMPK that sensitizes tumor cells to doxorubicin in vitro and in vivo, suggesting its potential as a therapeutic option.	22789537

Reference overview

Pub Med ID

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