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MABC608 Anti-dUTPase Antibody, clone 6A6.2

Anti-dUTPase Antibody, clone 6A6.2 is an antibody against dUTPase for use in Western Blotting, Immunocytochemistry.

Anti-dUTPase Antibody, clone 6A6.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: DUT UniProt Number: P33316
MABC608
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC608
DescriptionAnti-dUTPase Antibody, clone 6A6.2
Alternate Names
  • Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
  • dUTPase
  • dUTP pyrophosphatase
Background InformationDeoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23; also known as dUTPase, dUTP nucleotidohydrolase, or dUTP pyrophosphatase) is encoded by the DUT gene (Entrez Gene ID 1854) in human. dUTPase catalyzes the hydrolysis of dUTP to generate dUMP. This reaction serves to provide dUMP of thymine nucleotides synthesis needed for DNA replication. dUTP hydrolysis by dUTPase also prevents increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. The DUT gene encodes both mitochondrial and nuclear dATPase (DUT-M and DUT-N, respectively) via alternative splicing. DUT-N (164 aa, 17.7 kDa) corresponds to DUT-M (252 aa, 26.6 kDa) with the first 93 amino acid sequence replaced by MPCSE. Cat. No. MABC608, Anti-dUTPase, clone 6A6.2, is a purified mouse monoclonal antibody. A GST fusion protein containing the full-lenght human DUT-N (UniProt P33316) sequence was used as the immunogen. The binding epitope of clone 6A6.2 is not yet mapped.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-dUTPase Antibody, clone 6A6.2 is an antibody against dUTPase for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected dUTPase in HeLa and A431 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human dUTPase.
Clone6A6.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DUT
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~18 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 and Raji cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected dUTPase in 10 µg of A431 and Raji cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC608 04055977164053