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ABE1339 Sigma-AldrichAnti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated)

This Anti-dimethyl-BAF155 antibody is validated for use in WB, IP, ChIP, IH for the detection of dimethyl-BAF155.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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References

Research Category: Epigenetics & Nuclear Function, Apoptosis & Cancer Research Sub-Category: Chromatin Biology Gene Symbol: SMARCC1, BAF155 UniProt Number: Q92922

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP, ChIP, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABE1339
Description	Anti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated)
Alternate Names	BRG1-associated factor 155
Background Information	BAF155, also known as SWI/SNF complex subunit BRG1-associated factor 155, BAF155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, and encoded by the gene name SMARCC1 and BAF155, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). BAF155 has been implied to stimulate the ATPase activity of the catalytic subunit of the complex. BAF155 may also be involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. BAF155 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). BAF155 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas.

References

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 10% glycerol.
Quality Level	MQ100

Applications
Application	This Anti-dimethyl-BAF155 antibody is validated for use in WB, IP, ChIP, IH for the detection of dimethyl-BAF155.
Key Applications	Western Blotting Immunoprecipitation Chromatin Immunoprecipitation (ChIP) Immunohistochemistry
Application Notes	Western Blotting Analysis: A representative lot of this antibody detected methylated BAF155 in transfected MCF-7 cell lysate (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014) Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated methylated BAF155 from transfected SKOV3 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014) ChIP: A representative lot of this antibody chromatin immunoprecipitated BAF155 to COL1A2 and GADD45A genes in wildtype MCF-7 cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014) Immunohistochemical Analysis: A representative lot of this antibody detected methylated BAF155 protein in human breast tumor cells (Wang, L., et al. Cancer Cell 25, 21–36, January 13, 2014)

Biological Information
Immunogen	linear peptide corresponding to the C-terminus of Human BAF155 protein dimethylated at Arg 1064.
Epitope	C-terminus
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003065
Gene Symbol	SMARCC1 BAF155
Modifications	Methylation
Purification Method	Antigen Affinity Purified
UniProt Number	Q92922
Molecular Weight	~ 160 kDa observed. The ~160 kDa band has been confirmed to be the target protein BAF155 by mass spectrometry

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting on MCF-7 cell lysate Western Blotting Analysis: A 1:1000 dilution of this antibody detected dimethyl-BAF155 protein in 10 µg of MCF-7 cell lysate
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
ABE1339	04055977121575

Documentation

Anti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated) SDS

Title
Safety Data Sheet (SDS)

Anti-dimethyl-BAF155 Antibody (Arg1064, asymetrically di-methylated) Certificates of Analysis

Title	Lot Number
Anti-dimethyl-BAF155 (Arg1064, -2820727	2820727
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) - 3174767	3174767
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) - 3723493	3723493
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) - 3835340	3835340
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) - 4053774	4053774
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) -Q2488088	Q2488088
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) Polyclonal Antibody	2930959
Anti-dimethyl-BAF155 (Arg1064, asymetrically di-methylated) Polyclonal Antibody	3118125

References

Reference overview	Pub Med ID
CARM1 methylates chromatin remodeling factor BAF155 to enhance tumor progression and metastasis. Wang, L; Zhao, Z; Meyer, MB; Saha, S; Yu, M; Guo, A; Wisinski, KB; Huang, W; Cai, W; Pike, JW; Yuan, M; Ahlquist, P; Xu, W Cancer cell 25 21-36 2014 Show Abstract Coactivator-associated arginine methyltransferase 1 (CARM1), a coactivator for various cancer-relevant transcription factors, is overexpressed in breast cancer. To elucidate the functions of CARM1 in tumorigenesis, we knocked out CARM1 from several breast cancer cell lines using Zinc-Finger Nuclease technology, which resulted in drastic phenotypic and biochemical changes. The CARM1 KO cell lines enabled identification of CARM1 substrates, notably the SWI/SNF core subunit BAF155. Methylation of BAF155 at R1064 was found to be an independent prognostic biomarker for cancer recurrence and to regulate breast cancer cell migration and metastasis. Furthermore, CARM1-mediated BAF155 methylation affects gene expression by directing methylated BAF155 to unique chromatin regions (e.g., c-Myc pathway genes). Collectively, our studies uncover a mechanism by which BAF155 acquires tumorigenic functions via arginine methylation.	24434208

Reference overview

Pub Med ID

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