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ABE353 Anti-dimethyl Histone H2B Antibody (Pro1)

This Anti-dimethyl Histone H2B Antibody (Pro1) is validated for use in Western Blotting and Immunocytochemistry and Dot Blot for the detection of dimethyl Histone H2B (Pro1).

Anti-dimethyl Histone H2B Antibody (Pro1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology Gene Symbol: HIST1H2BC, H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK UniProt Number: P62807
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ABE353
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE353
DescriptionAnti-dimethyl Histone H2B Antibody (Pro1)
Alternate Names
  • Histone H2B type 1-C/E/F/G/
  • H2Bme2P1
  • Histone H2B.1 A
  • Histone H2B.a
  • H2B/a
  • Histone H2B.g
  • H2B/g
  • Histone H2B.h
  • Histone H2B.k
  • H2B.l
  • H2B/l
Background InformationHistone H2B is one of four histones that form a core octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability; and consequently, transcription, and DNA replication and repair. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide transcriptional complexes, for example, with access to DNA, potentially resulting in increased gene transcription. Lysine 120 of H2B (H2BK120) undergoes acetylation (by p300/CBP) and ubiquitination. Acetylation occurs first and is required for subsequent mono-ubiquitination. H2BK120 may function as a marker of “pre-active” chromatin, and is part of a complex regulatory system involving multiple histone modifications.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-dimethyl Histone H2B Antibody (Pro1) is validated for use in Western Blotting and Immunocytochemistry and Dot Blot for the detection of dimethyl Histone H2B (Pro1).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
Application NotesDot Blot Specificity Analysis:
Peptide containing various unmodified and modified Histone peptides were probed with Anti-dimethyl Histone H2B (Pro1) antibody at 0.1 ug/mL.

Immunocytochemistry Analysis:
A 10 µg/mL dilution from a representative lot detected Histone H2B dimethylated at proline 1 in A431, HeLa, and NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of Human dimethyl Histone H2B methylated at Pro1.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes Histone H2B dimethylated at Proline 1.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H2BC
  • H2BFL
  • HIST1H2BE
  • H2BFH
  • HIST1H2BF
  • H2BFG
  • HIST1H2BG
  • H2BFA
  • HIST1H2BI
  • H2BFK
Modifications
  • Methylation
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H2B dimethylated at proline 1 in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE353 04053252985225