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04-835-S Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, Trial Size, rabbit monoclonal

Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59 is a rabbit monoclonal antibody for detection of dimethyl-Histone H3 (Lys79) also known as H3K79me2, Histone H3 (di methyl K79) & has been validated in ChIP, WB, DB, Mplex.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H3F3A, H3F3B
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04-835-S
10 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, VrtWB, DB, Mplex, ChIP-seq, ChIPRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-835-S
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, Trial Size, rabbit monoclonal
Alternate Names
  • H3K79me2
  • Histone H3 (di methyl K79)
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Dimethylation of Lys79 on histone H3 is associated with active gene transcription and is a marker for euchromatin. Histone H3 is methylated on Lys79 by the histone methyltransferase Dot1L.
References
Product Information
FormatCulture Supernatant
PresentationCulture supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59 is a rabbit monoclonal antibody for detection of dimethyl-Histone H3 (Lys79) also known as H3K79me2, Histone H3 (di methyl K79) & has been validated in ChIP, WB, DB, Mplex.
Key Applications
  • Western Blotting
  • Dot Blot
  • Multiplexing
  • ChIP-seq
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 4 µL of Negative Control Supernatant, or 4 µL of Anti-dimethyl-Histone H3 (Lys79) and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of dimethyl-Histone H3 (Lys79) associated DNA fragments was verified by qPCR using Control Primers
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Chromatin Immunoprecipitation: A representative lot of this antibody has been shown by an independent laboratory to work in ChIP.

ChIP-Seq Analysis:
A representative lot of this antibody was used by an independent laboratory for ChIP-Seq. See Egelhofer, T.A., et al. (2011). See Easwaran, H., et al. (2012).See Suzuki, H., et al. (2011).

Dot Blotting: Specificity of a representative lot confirmed by the ability of a 1:2500 dilution of the antibody to recognize peptides corresponding to regions of histone H3 with various modifications

Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-835-S, Anti-dimethyl Histone H3 (Lys79) Antibody, clone NL59 (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system..
Biological Information
ImmunogenKLH-conjugated synthetic peptide containing the sequence DFme2KTD corresponding to dimethyl-lysine at position 79 of histone H3. The immunizing sequence is identical in human, mouse, rat and yeast. A C-terminal cysteine was added to facilitate conjugation.
EpitopeLys79
CloneNL59
HostRabbit
SpecificityRecognizes dimethyl-histone H3 (Lys79).
IsotypeIgG
Species Reactivity
  • Human
  • Vertebrates
Species Reactivity NoteBroad species cross-reactivity expected due to sequence homology
Antibody TypeMonoclonal Antibody
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Gene Symbol
  • H3F3A
  • H3F3B
Modifications
  • Methylation
UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Molecular Weight17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot of acid extracted proteins from HeLa cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size10 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-835-S 04053252929878

Documentation

Anti-dimethyl-Histone H3 (Lys79) Antibody, clone NL59, Trial Size, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-dimethyl Histone H3 (Lys79), clone NL59, Rabbit Monoclonal Trial Size - 3184780 3184780
Anti-dimethyl Histone H3 (Lys79), clone NL59, Rabbit Monoclonal Trial Size - 3384063 3384063
Anti-dimethyl Histone H3 (Lys79), clone NL59,Rabbit Monoclonal Trial Size - 3739775 3739775
Anti-dimethyl Histone H3 (Lys79), clone NL59,Rabbit Monoclonal Trial Size - 4018522 4018522
Anti-dimethyl Histone H3 (Lys79), clone NL59,Rabbit Monoclonal Trial Size - Q2290322 Q2290322
Anti-dimethyl Histone H3 (Lys79), clone NL59,Rabbit Monoclonal Trial Size Monoclonal Antibody 2919629