Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

07-1522 Anti-eIF3A Antibody

Use Anti-eIF3A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect eIF3A also known as Eukaryotic translation initiation factor 3 subunit A.

Anti-eIF3A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: EIF3A, EIF3S10, KIAA0139 UniProt Number: Q14152
07-1522
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Chp, Eq, M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1522
DescriptionAnti-eIF3A Antibody
Alternate Names
  • Eukaryotic translation initiation factor 3 subunit A
Background InformationeIF3a is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which coordinates a myriad of protein-protein interactions that mediates the translation initiation process in eukaryotes. In particular, the eIF-3 complex is known to stimulate the recruitment of eIF-1, eIF-1a, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex is known to recruit mRNA to the 43SPIC complex, which then initiates scanning of mRNA for the AUG start codon. In addition, the eIF-3 complex is required for disassembly and recycling of post-termination ribosomal complexes and prevents the premature joining of the 40S and 60S ribosomal subunits prior to initiation.
References
Product Information
FormatAffinity Purified
Control
  • HEK293T cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationUse Anti-eIF3A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect eIF3A also known as Eukaryotic translation initiation factor 3 subunit A.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to eIF3A.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Chimpanzee
  • Horse
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Chimpanzee, Equine, Mouse, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EIF3A
  • EIF3S10
  • KIAA0139
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

SUBUNIT STRUCTURE: Interacts with EIF4G1. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.

SUBCELLULAR LOCATION: Cytoplasm.
Molecular Weight~167 kDa observed. An uncharacterized band may be observed at a molecular weight of ~97 kDa in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293T cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected eIF3A in HEK293T cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1522 04053252423635